BMRB: 4447

Backbone assignment of the 19kDa translationally controlled tumor-associated protein p23fyp from Schizosaccharomyces pombe

Authors

Baxter, N.J., Thaw, P., Higgins, L.D., Sedelnikova, S.E., Bramley, A.L., Price, C., Waltho, J.P., Craven, C.

Assembly

p23fyp

Entity

1. p23fyp (polymer, Thiol state: all free), 168 monomers, 19048.30 Da Detail

MLLYKDVISG DELVSDAYDL KEVDDIVYEA DCQMVTVKQG GDVDIGANPS AEDAEENAEE GTETVNNLVY SFRLSPTSFD KKSYMSYIKG YMKAIKARLQ ESNPERVPVF EKNAIGFVKK ILANFKDYDF YIGESMDPDA MVVLMNYRED GITPYMIFFK DGLVSEKF

Formula weight

19048.3 Da

Source organism

Schizosaccharomyces pombe

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 55.2 %, Completeness (bb): 98.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	55.2 % (1086 of 1966)	40.8 % (416 of 1019)	65.9 % (510 of 774)	92.5 % (160 of 173)
Backbone	98.5 % (981 of 996)	98.2 % (334 of 340)	98.8 % (488 of 494)	98.1 % (159 of 162)
Sidechain	22.4 % (253 of 1128)	11.6 % (79 of 679)	39.7 % (174 of 438)	0.0 % (0 of 11)
Aromatic	3.4 % (6 of 178)	4.5 % (4 of 89)	2.2 % (2 of 89)
Methyl	14.5 % (25 of 172)	9.3 % (8 of 86)	19.8 % (17 of 86)

1. p23fyp

MLLYKDVISG DELVSDAYDL KEVDDIVYEA DCQMVTVKQG GDVDIGANPS AEDAEENAEE GTETVNNLVY SFRLSPTSFD KKSYMSYIKG YMKAIKARLQ ESNPERVPVF EKNAIGFVKK ILANFKDYDF YIGESMDPDA MVVLMNYRED GITPYMIFFK DGLVSEKF

Show sample condition

Sample

Temperature 308 (±1) K, pH 5.1 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	p23fyp	[U-13C; U-15N]		1 mM
2	sodium acetate	[U-2H]		20 mM
3	sodium chloride			250 mM
4	sodium azide			5 mM
5	PMSF			1 mM

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LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
13C	TSP	methyl protons	0.0 ppm	internal	indirect	25.14495
15N	TSP	methyl protons	0.0 ppm	internal	indirect	10.13291

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LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
13C	TSP	methyl protons	0.0 ppm	internal	indirect	25.14495
15N	TSP	methyl protons	0.0 ppm	internal	indirect	10.13291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
13C	TSP	methyl protons	0.0 ppm	internal	indirect	25.14495
15N	TSP	methyl protons	0.0 ppm	internal	indirect	10.13291

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LACS Plot; CO

Referencing offset: 0.09 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
13C	TSP	methyl protons	0.0 ppm	internal	indirect	25.14495
15N	TSP	methyl protons	0.0 ppm	internal	indirect	10.13291

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Protein Blocks Logo

Calculated from 15 models in PDB: 1H7Y, Strand ID: A Detail

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Release date

2000-03-13

Citation

Letter to the Editor: Backbone assignment of the 19kDa translationally controlled tumor-associated protein p23 fyp from Schizosaccharomyces pombe
Baxter, N.J., Thaw, P., Higgins, L.D., Sedelnikova, S.E., Bramley, A.L., Price, C., Waltho, J.P., Craven, C.
J. Biomol. NMR (2000), 16, 83-84, PubMed , DOI:

Related entities 1. p23fyp, : 1 : 3 : 112 entities Detail

Experiments performed 7 experiments Detail