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BMRB: 4455

1CI5

Glycan-free mutant adhesion domain of human CD58 (LFA-3)

Authors

Sun, Z.J., Dotsch, V., Kim, M., Li, J., Reinherz, E.L., Wagner, G.

Assembly

CD58 adhesion domain, 1dCD58

Entity

1. CD58 adhesion domain, 1dCD58 (polymer, Thiol state: not present), 95 monomers, 10991.08 Da Detail

SSQQIYGVKY GNVTFHVPSN QPLKEVLWKK QKDKVAELEN SEFRAFSSFK NRVYLDTKSG SLTIYNLTSS DEDEYEMESP NITDSMKFFL YVGES

Formula weight

10991.08 Da

Source organism

Exptl. method

Refine. method

DISTANCE GEOMETRY

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.7 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.7 % (1028 of 1146)	90.6 % (542 of 598)	86.5 % (385 of 445)	98.1 % (101 of 103)
Backbone	98.4 % (555 of 564)	99.0 % (189 of 191)	98.2 % (276 of 281)	97.8 % (90 of 92)
Sidechain	83.8 % (564 of 673)	86.7 % (353 of 407)	78.4 % (200 of 255)	100.0 % (11 of 11)
Aromatic	37.1 % (46 of 124)	62.9 % (39 of 62)	9.8 % (6 of 61)	100.0 % (1 of 1)
Methyl	100.0 % (82 of 82)	100.0 % (41 of 41)	100.0 % (41 of 41)

1. 1dCD58

SSQQIYGVKY GNVTFHVPSN QPLKEVLWKK QKDKVAELEN SEFRAFSSFK NRVYLDTKSG SLTIYNLTSS DEDEYEMESP NITDSMKFFL YVGES

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	1dCD58	[U-13C; U-15N]		0.2 ~ 0.6 mM
2	NaPO4			10 mM
3	D2O			10 %

LACS Plot; CA

Referencing offset: -0.44 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.44 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 0.95 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1CI5, Strand ID: A Detail

Release date

1999-11-10

Citation 1

Functional glycan-free adhesion domain of human cell surface receptor CD58: design, production and NMR studies
Sun, Z.J., Dotsch, V., Kim, M., Li, J., Reinherz, E.L., Wagner, G.
EMBO J. (1999), 18, 2941-2949, PubMed 10357807 , DOI 10.1093/emboj/18.11.2941 , Abstract

Show more 1 citaion

Citation 2

No title is available
Wishart, D.

Hide non-primary 1 citaion

Related entities 1. CD58 adhesion domain, 1dCD58, : 1 : 1 : 17 entities Detail

Interaction partners 1. CD58 adhesion domain, 1dCD58, : 4 interactors Detail

More details for 4 interactors identified by 3 citations

Experiments performed 10 experiments Detail

1 NOESY

Instrument

Bruker AMX - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	1dCD58	[U-13C; U-15N]		0.2 ~ 0.6 mM
2	NaPO4			10 mM
3	D2O			10 %

2 NOESY-HSQC

Instrument

Bruker AMX - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	1dCD58	[U-13C; U-15N]		0.2 ~ 0.6 mM
2	NaPO4			10 mM
3	D2O			10 %

3 HNCA

Instrument

Bruker AMX - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	1dCD58	[U-13C; U-15N]		0.2 ~ 0.6 mM
2	NaPO4			10 mM
3	D2O			10 %

4 CBCA(CO)NH

Instrument

Bruker AMX - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	1dCD58	[U-13C; U-15N]		0.2 ~ 0.6 mM
2	NaPO4			10 mM
3	D2O			10 %

5 HBHA(CBCACO)NH

Instrument

Bruker AMX - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	1dCD58	[U-13C; U-15N]		0.2 ~ 0.6 mM
2	NaPO4			10 mM
3	D2O			10 %

6 HCCH-TOCSY

Instrument

Bruker AMX - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	1dCD58	[U-13C; U-15N]		0.2 ~ 0.6 mM
2	NaPO4			10 mM
3	D2O			10 %

7 HNHA

Instrument

Bruker AMX - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	1dCD58	[U-13C; U-15N]		0.2 ~ 0.6 mM
2	NaPO4			10 mM
3	D2O			10 %

8 HNHB

Instrument

Bruker AMX - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	1dCD58	[U-13C; U-15N]		0.2 ~ 0.6 mM
2	NaPO4			10 mM
3	D2O			10 %

9 HNCO

Instrument

Bruker AMX - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	1dCD58	[U-13C; U-15N]		0.2 ~ 0.6 mM
2	NaPO4			10 mM
3	D2O			10 %

10 HN(CA)CO

Instrument

Bruker AMX - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	1dCD58	[U-13C; U-15N]		0.2 ~ 0.6 mM
2	NaPO4			10 mM
3	D2O			10 %

nullKeywords adhesion glycoprotein, cell surface receptor, immunoglobulin superfamily V-set domain