BMRB: 4473

The molecular basis for Protein Kinase A anchoring revealed by solution NMR

Authors

Newlon, M.G., Roy, M., Morikis, D., Hausken, Z.E., Coghlan, V., Scott, J.D., Jennings, P.A.

Assembly

Camp dependent protein kinase type ii regulatory chain

Entity

1. Camp dependent protein kinase type ii regulatory chain (polymer, Thiol state: not present), 46 monomers, 5391.123 × 2 Da Detail

HMGHIQIPPG LTELLQGYTV EVLRQQPPDL VDFAVEYFTR LREARR

Total weight

10782.246 Da

Max. entity weight

5391.123 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY-SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 80.1 %, Completeness (bb): 83.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	80.1 % (448 of 559)	84.6 % (248 of 293)	72.3 % (159 of 220)	89.1 % (41 of 46)
Backbone	83.2 % (223 of 268)	98.9 % (90 of 91)	68.1 % (92 of 135)	97.6 % (41 of 42)
Sidechain	80.2 % (268 of 334)	78.2 % (158 of 202)	85.9 % (110 of 128)	0.0 % (0 of 4)
Aromatic	40.9 % (18 of 44)	45.5 % (10 of 22)	36.4 % (8 of 22)
Methyl	98.3 % (57 of 58)	100.0 % (29 of 29)	96.6 % (28 of 29)

1. Camp dependent protein kinase type ii regulatory chain

HMGHIQIPPG LTELLQGYTV EVLRQQPPDL VDFAVEYFTR LREARR

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 4.0

#	Name	Isotope labeling	Type	Concentration
1	Camp dependent protein kinase type ii regulatory chain

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Protein Blocks Logo

Calculated from 17 models in PDB: 1R2A, Strand ID: A, B Detail

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Release date

2000-05-24

Citation

The molecular basis for protein kinase A anchoring revealed by solution NMR
NEWLON, M.G., ROY, M., MORIKIS, D., HAUSKEN, Z.E., COGHLAN, V., SCOTT, J.D., JENNINGS, P.A.
Nat. Struct. Biol. (1999), 6, 222-227, PubMed 10074940 , DOI 10.1038/6663 , Abstract

Related entities 1. Camp dependent protein kinase type ii regulatory chain, : 1 : 4 : 6 : 66 entities Detail

Interaction partners 1. Camp dependent protein kinase type ii regulatory chain, : 14 interactors Detail

Experiments performed 7 experiments Detail