BMRB: 4486

METHANE MONOOXYGENASE COMPONENT B

Authors

CHANG, S.L., WAllAR, B.J., LIPSCOMB, J.D., MAYO, K.H.

Assembly

METHANE MONOOXYGENASE REGULATORY PROTEIN B

Entity

1. METHANE MONOOXYGENASE REGULATORY PROTEIN B (polymer, Thiol state: not present), 138 monomers, 14882.76 Da Detail

MSSAHNAYNA GIMQKTGKAF ADEFFAEENQ VVHESNAVVL VLMKSDEIDA IIEDIVLKGG KAKNPSIVVE DKAGFWWIKA DGAIEIDAAE AGELLGKPFS VYDLLINVSS TVGRAYTLGT KFTITSELMG LDRALTDI

Formula weight

14882.76 Da

Source organism

Methylosinus trichosporium

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 66.6 %, Completeness (bb): 77.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	66.6 % (1035 of 1554)	69.2 % (548 of 792)	59.1 % (364 of 616)	84.2 % (123 of 146)
Backbone	77.7 % (640 of 824)	88.8 % (253 of 285)	65.8 % (265 of 403)	89.7 % (122 of 136)
Sidechain	59.6 % (511 of 857)	58.2 % (295 of 507)	63.2 % (215 of 340)	10.0 % (1 of 10)
Aromatic	7.8 % (9 of 116)	8.6 % (5 of 58)	7.1 % (4 of 56)	0.0 % (0 of 2)
Methyl	74.5 % (137 of 184)	80.4 % (74 of 92)	68.5 % (63 of 92)

1. METHANE MONOOXYGENASE REGULATORY PROTEIN B

MSSAHNAYNA GIMQKTGKAF ADEFFAEENQ VVHESNAVVL VLMKSDEIDA IIEDIVLKGG KAKNPSIVVE DKAGFWWIKA DGAIEIDAAE AGELLGKPFS VYDLLINVSS TVGRAYTLGT KFTITSELMG LDRALTDI

Show sample condition

Sample

Temperature 313 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	METHANE MONOOXYGENASE REGULATORY PROTEIN B			0.0 ~ 0.0 mM

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LACS Plot; CA

Referencing offset: -0.31 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: -0.31 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.14 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 15 models in PDB: 2MOB, Strand ID: A Detail

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Release date

2000-06-16

Citation

Solution structure of component B from methane monooxygenase derived through heteronuclear NMR and molecular modeling
CHANG, S.L., WAllAR, B.J., LIPSCOMB, J.D., MAYO, K.H.
Biochemistry (1999), 38, 5799-5812, PubMed 10231531 , DOI 10.1021/bi982992f , Abstract

Related entities 1. METHANE MONOOXYGENASE REGULATORY PROTEIN B, : 1 : 8 : 4 : 6 entities Detail