BMRB: 4487

Putative ancestral protein encoded by a single sequence repeat of the multidomain proteinase inhibitor from nicotiana alata

Authors

Scanlon, M.J., Lee, M.C.S., Anderson, M.A., Craik, D.J.

Assembly

API

Entity

1. API (polymer, Thiol state: all disulfide bound), 54 monomers, 5919.682 Da Detail

MKACTLNCDP RIAYGVCPRS EEKKNDRICT NCCAGTKGCK YFSDDGTFVC EGES

Formula weight

5919.682 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	0:CYS4:SG	1:CYS33:SG
2	disulfide	sing	0:CYS8:SG	1:CYS29:SG
3	disulfide	sing	0:CYS17:SG	1:CYS39:SG
4	disulfide	sing	0:CYS32:SG	1:CYS50:SG

Source organism

Nicotiana alata

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 97.0 %, Completeness (bb): 98.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	97.0 % (296 of 305)	97.0 % (296 of 305)
Backbone	98.2 % (109 of 111)	98.2 % (109 of 111)
Sidechain	96.4 % (187 of 194)	96.4 % (187 of 194)
Aromatic	100.0 % (18 of 18)	100.0 % (18 of 18)
Methyl	100.0 % (17 of 17)	100.0 % (17 of 17)

1. API

MKACTLNCDP RIAYGVCPRS EEKKNDRICT NCCAGTKGCK YFSDDGTFVC EGES

Show sample condition

Sample

Pressure 1 atm, Temperature 313 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
1	API			0.0 ~ 0.0 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1CE3, Strand ID: A Detail

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Release date

2000-06-16

Citation

Structure of a putative ancestral protein encoded by a single sequence repeat from a multidomain proteinase inhibitor gene fro Nicotiana alata
Scanlon, M.J., Lee, M.C.S., Anderson, M.A., Craik, D.J.
Struc. Fold. Des. (1999), 7, 793-802, PubMed , DOI:

Related entities 1. API, : 1 : 1 : 31 entities Detail

Experiments performed 4 experiments Detail