BMRB: 4488

DNA decamer duplex containing T-T (6-4) photoadduct

Authors

Lee, J.H., Hwang, G.-S., Choi, B.-S.

Assembly

DNA decamer

Entity

1. DNA decamer strand 1 (polymer, Thiol state: not present), 10 monomers, 3085.968 Da Detail

CGCAXTACGC

2. DNA decamer strand 2 (polymer, Thiol state: not present), 10 monomers, 3163.999 Da Detail

GCGTGATGCG

Total weight

6249.967 Da

Max. entity weight

3163.999 Da

Entity Connection

covalent 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	covalent	sing	1:64T5:C6	1:DT6:C4

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 45.0 %, Completeness: 38.2 %, Completeness (bb): 33.8 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	38.2 % (71 of 186)	38.2 % (71 of 186)
Suger, PO4	33.8 % (45 of 133)	33.8 % (45 of 133)
Nucleobase	49.1 % (26 of 53)	49.1 % (26 of 53)
Aromatic	44.7 % (17 of 38)	44.7 % (17 of 38)
Methyl	33.3 % (1 of 3)	33.3 % (1 of 3)

1. DNA decamer strand 1

CGCAXTACGC

2. DNA decamer strand 2

GCGTGATGCG

Show sample condition

Sample

Temperature 274 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
1	DNA decamer strand 1			0.0 ~ 0.0 mM
2	DNA decamer strand 2			0.0 ~ 0.0 mM

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Chem. Shift Complete2

Sequence coverage: 50.0 %, Completeness: 39.5 %, Completeness (bb): 35.7 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	39.5 % (147 of 372)	39.5 % (147 of 372)
Suger, PO4	35.7 % (95 of 266)	35.7 % (95 of 266)
Nucleobase	49.1 % (52 of 106)	49.1 % (52 of 106)
Aromatic	48.7 % (37 of 76)	48.7 % (37 of 76)
Methyl	50.0 % (3 of 6)	50.0 % (3 of 6)

1. DNA decamer strand 1

CGCAXTACGC

2. DNA decamer strand 2

GCGTGATGCG

Show sample condition

Sample

Temperature 274 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
1	DNA decamer strand 1			0.0 ~ 0.0 mM
2	DNA decamer strand 2			0.0 ~ 0.0 mM

Hide sample codition

Release date

2000-08-03

Citation

Solution structure of a DNA decamer duplex containing the stable 3' T.G base pair of the pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct]: implications for the highly specific 3' T --> C transition of the (6-4) adduct
Lee, J.H., Hwang, G.-S., Choi, B.-S.
Proc. Natl. Acad. Sci. U. S. A. (1999), 96, 6632-6636, PubMed 10359763 , DOI: , Abstract

Experiments performed 1 experiments Detail