BMRB: 4491

Solution structure of the apo EH1 domain of mouse Eps15

Authors

Whitehead, B., Tessari, M., Carotenuto, A., van Bergen en Henegouwen, P.M.P., Vuister, G.W.

Assembly

Eps15

Entity

1. Eps15 (polymer, Thiol state: not reported), 120 monomers, 12631.32 Da Detail

MAAAAQLSLT QLSSGNPVYE KYYRQVEAGN TGRVLALDAA AFLKKSGLPD LILGKIWDLA DTDGKGVLSK QEFFVALRLV ACAQNGLEVS LSSLSLAVPP PRFHDSSSPL LTSGPSVAEL

Formula weight

12631.32 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

TAD

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.7 %, Completeness (bb): 97.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.7 % (1228 of 1339)	89.1 % (612 of 687)	93.6 % (496 of 530)	98.4 % (120 of 122)
Backbone	97.2 % (686 of 706)	97.5 % (236 of 242)	96.6 % (339 of 351)	98.2 % (111 of 113)
Sidechain	87.4 % (650 of 744)	84.5 % (376 of 445)	91.4 % (265 of 290)	100.0 % (9 of 9)
Aromatic	60.0 % (48 of 80)	57.5 % (23 of 40)	61.5 % (24 of 39)	100.0 % (1 of 1)
Methyl	97.5 % (158 of 162)	97.5 % (79 of 81)	97.5 % (79 of 81)

1. Eps15

MAAAAQLSLT QLSSGNPVYE KYYRQVEAGN TGRVLALDAA AFLKKSGLPD LILGKIWDLA DTDGKGVLSK QEFFVALRLV ACAQNGLEVS LSSLSLAVPP PRFHDSSSPL LTSGPSVAEL

Show sample condition

Sample #1

Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	Eps15	[U-15N]		1.0 mM
2	potassium phosphate			100 mM
3	sodium chloride			100 mM
4	H2O			90 %
5	D2O			10 %

Sample #2

Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
6	Eps15	[U-15N; U-13C]		1.0 mM
7	potassium phosphate			100 mM
8	sodium chloride			100 mM
9	H2O			90 %
10	D2O			10 %

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LACS Plot; CA

Referencing offset: 0.13 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	H2O	proton	43.2 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	0.0
15N	H2O	proton	118.04 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.13 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	H2O	proton	43.2 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	0.0
15N	H2O	proton	118.04 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	H2O	proton	43.2 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	0.0
15N	H2O	proton	118.04 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.22 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	H2O	proton	43.2 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	0.0
15N	H2O	proton	118.04 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 30 models in PDB: 1QJT, Strand ID: A Detail

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Release date

2000-06-16

Citation

Sequence-specific 1H, 13C and 15N assignment of the EH1 domain of mouse Eps15
Whitehead, B., Tessari, M., Versteeg, H.H., van Delft, S., van Bergen en Henegouwen, P.M.P., Vuister, G.W.
J. Biomol. NMR (1998), 12, 465-466, PubMed 9835057 , DOI: , Abstract

Related entities 1. Eps15, : 1 : 2 : 28 entities Detail

Interaction partners 1. Eps15, : 14 interactors Detail