BMRBj - BMREntryMaster

Found 1 Document (2.882 seconds)

BMRB: 4492

1JBA

Unmyristoylated GCAP-2 with three calcium ions bound

Authors

Ames, J.B., Dizhoor, A.M., Ikura, M., Palczewski, K., Stryer, L.

Assembly

guanylate cyclase activating protein 2

Entity

1. guanylate cyclase activating protein 2 (polymer, Thiol state: not reported), 204 monomers, 23727.59 Da Detail

MGQQFSWEEA EENGAVGAAD AAQLQEWYKK FLEECPSGTL FMHEFKRFFK VPDNEEATQY VEAMFRAFDT NGDNTIDFLE YVAALNLVLR GTLEHKLKWT FKIYDKDRNG CIDRQELLDI VESIYKLKKA CSVEVEAEQQ GKLLTPEEVV DRIFLLVDEN GDGQLSLNEF VEGARRDKWV MKMLQMDLNP SSWISQQRRK SAMF

2. CA (non-polymer), 40.078 × 3 Da

Total weight

23847.824 Da

Max. entity weight

23727.59 Da

Source organism

Bos taurus

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.0 %, Completeness: 62.2 %, Completeness (bb): 94.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	62.2 % (1533 of 2465)	56.2 % (724 of 1288)	65.2 % (620 of 951)	83.6 % (189 of 226)
Backbone	94.6 % (1150 of 1216)	94.2 % (391 of 415)	95.0 % (571 of 601)	94.0 % (188 of 200)
Sidechain	39.5 % (569 of 1442)	38.1 % (333 of 873)	43.3 % (235 of 543)	3.8 % (1 of 26)
Aromatic	10.9 % (26 of 238)	10.9 % (13 of 119)	10.5 % (12 of 114)	20.0 % (1 of 5)
Methyl	31.4 % (64 of 204)	31.4 % (32 of 102)	31.4 % (32 of 102)

1. guanylate cyclase activating protein 2

Show sample condition

Sample #1

Pressure 1 atm, Temperature 318 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	guanylate cyclase activating protein 2	[U-15N]	1 mM
2	KCl		50 mM
3	CaCl2		10 mM
4	[2H10]dithiothreitol		10 mM
5	[2H28]octyl-glucoside		25 mM

Sample #2

Pressure 1 atm, Temperature 318 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	guanylate cyclase activating protein 2	[U-13C; U-15N]	1 mM
7	KCl		50 mM
8	CaCl2		10 mM
9	[2H10]dithiothreitol		10 mM
10	[2H28]octyl-glucoside		25 mM

LACS Plot; CA

Referencing offset: -0.36 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: -0.36 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Referencing offset: -0.27 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 22 models in PDB: 1JBA, Strand ID: A Detail

Release date

2000-06-15

Citation

Three-dimensional structure of guanylyl cyclase activating protein-2, a calcium-sensitive modulator of photoreceptor guanylyl cyclases
Ames, J.B., Dizhoor, A.M., Ikura, M., Palczewski, K., Stryer, L.
J. Biol. Chem. (1999), 274, 19329-19337, PubMed 10383444 , DOI: , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P51177 released on 1996-10-01
Title GUC1B_BOVIN Entity Guanylyl cyclase-activating protein 2

Related entities 1. guanylate cyclase activating protein 2, : 1 : 1 : 223 entities Detail

Interaction partners 1. guanylate cyclase activating protein 2, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 5 experiments Detail

1 HNCO

Instrument

Model Varian Unity-Plus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 318 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	guanylate cyclase activating protein 2	[U-15N]	1 mM
2	KCl		50 mM
3	CaCl2		10 mM
4	[2H10]dithiothreitol		10 mM
5	[2H28]octyl-glucoside		25 mM

Sample #2

Pressure 1 atm, Temperature 318 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	guanylate cyclase activating protein 2	[U-13C; U-15N]	1 mM
7	KCl		50 mM
8	CaCl2		10 mM
9	[2H10]dithiothreitol		10 mM
10	[2H28]octyl-glucoside		25 mM

2 HNCACB

Instrument

Model Varian Unity-Plus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 318 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	guanylate cyclase activating protein 2	[U-15N]	1 mM
2	KCl		50 mM
3	CaCl2		10 mM
4	[2H10]dithiothreitol		10 mM
5	[2H28]octyl-glucoside		25 mM

Sample #2

Pressure 1 atm, Temperature 318 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	guanylate cyclase activating protein 2	[U-13C; U-15N]	1 mM
7	KCl		50 mM
8	CaCl2		10 mM
9	[2H10]dithiothreitol		10 mM
10	[2H28]octyl-glucoside		25 mM

3 CBCACONH

Instrument

Model Varian Unity-Plus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 318 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	guanylate cyclase activating protein 2	[U-15N]	1 mM
2	KCl		50 mM
3	CaCl2		10 mM
4	[2H10]dithiothreitol		10 mM
5	[2H28]octyl-glucoside		25 mM

Sample #2

Pressure 1 atm, Temperature 318 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	guanylate cyclase activating protein 2	[U-13C; U-15N]	1 mM
7	KCl		50 mM
8	CaCl2		10 mM
9	[2H10]dithiothreitol		10 mM
10	[2H28]octyl-glucoside		25 mM

4 NOESY

Instrument

Model Varian Unity-Plus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 318 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	guanylate cyclase activating protein 2	[U-15N]	1 mM
2	KCl		50 mM
3	CaCl2		10 mM
4	[2H10]dithiothreitol		10 mM
5	[2H28]octyl-glucoside		25 mM

Sample #2

Pressure 1 atm, Temperature 318 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	guanylate cyclase activating protein 2	[U-13C; U-15N]	1 mM
7	KCl		50 mM
8	CaCl2		10 mM
9	[2H10]dithiothreitol		10 mM
10	[2H28]octyl-glucoside		25 mM

5 TOCSY

Instrument

Model Varian Unity-Plus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 318 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	guanylate cyclase activating protein 2	[U-15N]	1 mM
2	KCl		50 mM
3	CaCl2		10 mM
4	[2H10]dithiothreitol		10 mM
5	[2H28]octyl-glucoside		25 mM

Sample #2

Pressure 1 atm, Temperature 318 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	guanylate cyclase activating protein 2	[U-13C; U-15N]	1 mM
7	KCl		50 mM
8	CaCl2		10 mM
9	[2H10]dithiothreitol		10 mM
10	[2H28]octyl-glucoside		25 mM

nullKeywords EF-hand, calcium-binding protein, guanylyl cyclase regulation

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (2.882 seconds)

BMRB: 4492

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. guanylate cyclase activating protein 2, : 1 : 1 : 223 entities Detail

Interaction partners 1. guanylate cyclase activating protein 2, : 2 interactors Detail

Experiments performed 5 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2