BMRB: 4494

Solution Structure and Backbone Dynamics of Human Long-[Arg3]Insulin-Like Growth Factor 1

Authors

Laajoki, L.G., Francis, G.L., Wallace, J.C., Carver, J.A., Keniry, M.A.

Assembly

IGF-I

Entity

1. IGF-I (polymer, Thiol state: all disulfide bound), 83 monomers, 9117.500 Da Detail

MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

Formula weight

9117.5 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS31:SG	1:CYS74:SG
2	disulfide	sing	1:CYS19:SG	1:CYS61:SG
3	disulfide	sing	1:CYS60:SG	1:CYS65:SG

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY AND MOLECULAR DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.3 %, Completeness (bb): 99.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	88.3 % (505 of 572)	87.2 % (429 of 492)	95.0 % (76 of 80)
Backbone	99.6 % (241 of 242)	99.4 % (165 of 166)	100.0 % (76 of 76)
Sidechain	80.0 % (264 of 330)	81.0 % (264 of 326)	0.0 % (0 of 4)
Aromatic	40.5 % (17 of 42)	40.5 % (17 of 42)
Methyl	94.4 % (34 of 36)	94.4 % (34 of 36)

1. IGF-I

MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

Show sample condition

Sample

Temperature 303 K, pH 3.0

#	Name	Isotope labeling	Type	Concentration
1	IGF-I			1.0 mM

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Protein Blocks Logo

Calculated from 15 models in PDB: 3LRI, Strand ID: A Detail

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Release date

1999-12-05

Citation

Solution Structure and Backbone Dynamics of Human Long-[Arg3]-Insulin Like Growth factor-1
Laajoki, L.G., Francis, G.L., Wallace, J.C., Carver, J.A., Keniry, M.A.
J. Biol. Chem. (2000), 275, 10009-10015, PubMed , DOI:

Related entities 1. IGF-I, : 1 : 21 : 85 entities Detail

Interaction partners 1. IGF-I, : 9 interactors Detail

Experiments performed 9 experiments Detail