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Found 1 Document (2.051 seconds)

BMRB: 4496

1OM2

Solution nmr structure of the mitochondrial protein import receptor Tom20 from rat in a complex with a presequence peptide derived from rat aldehyde dehydrogenase (ALDH)

Authors

Abe, Y., Shodai, T., Muto, T., Mihara, K., Torii, H., Nishikawa, S., Endo, T., Kohda, D.

Assembly

Tom20

Entity

1. Tom20 (polymer, Thiol state: all free), 95 monomers, 10454.84 Da Detail

RAGLSKLPDL KDAEAVQKFF LEEIQLGEEL LAQGDYEKGV DHLTNAIAVC GQPQQLLQVL QQTLPPPVFQ MLLTKLPTIS QRIVSAQSLG EDDVE

Formula weight

10454.84 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS FOLLOWED BY RESTRAINED ENERGY MINIMIZATION WITH AMBER FORCE FIELD

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.5 %, Completeness (bb): 96.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.5 % (1076 of 1115)	97.6 % (568 of 582)	94.9 % (409 of 431)	97.1 % (99 of 102)
Backbone	96.8 % (540 of 558)	97.4 % (185 of 190)	96.4 % (269 of 279)	96.6 % (86 of 89)
Sidechain	96.4 % (623 of 646)	97.7 % (383 of 392)	94.2 % (227 of 241)	100.0 % (13 of 13)
Aromatic	81.0 % (34 of 42)	100.0 % (21 of 21)	61.9 % (13 of 21)
Methyl	96.9 % (126 of 130)	96.9 % (63 of 65)	96.9 % (63 of 65)

1. Tom20

RAGLSKLPDL KDAEAVQKFF LEEIQLGEEL LAQGDYEKGV DHLTNAIAVC GQPQQLLQVL QQTLPPPVFQ MLLTKLPTIS QRIVSAQSLG EDDVE

Show sample condition

Sample

Pressure 1 atm, Temperature 303 K, pH 6.8, Details 0.5 mM For structure determination 1 mM for 1H-15N heteronuclear experiments 0.2 mM for titration experiments

#	Name	Isotope labeling	Type	Concentration
1	Tom20	[U-13C; U-15N]		0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.09 ppm, Outliers: 9 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1OM2, Strand ID: A Detail

Release date

2000-03-22

Citation

Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20
Abe, Y., Shodai, T., Muto, T., Mihara, K., Torii, H., Nishikawa, S., Endo, T., Kohda, D.
Cell (2000), 100, 551-560, PubMed 10721992 , DOI: , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: Q62760 released on 1997-11-01
    Title TOM20_RAT Entity Mitochondrial import receptor subunit TOM20 homolog
  Swiss-Prot: P11884 released on 1989-10-01
    Title ALDH2_RAT Entity Aldehyde dehydrogenase, mitochondrial

Related entities 1. Tom20, : 1 : 3 : 15 entities Detail

Interaction partners 1. Tom20, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 14 experiments Detail

1 HNCA, HN(CO)CA