BMRB: 4506

Solution structure of carnobacteriocin B2

Authors

Wang, Y., Henz, M.E., Gallagher, N.L.F., Chai, S., Yan, L.Z., Gibbs, A.C., Stiles, M.E., Wishart, D.S., Vederas, J.C.

Assembly

type IIA bacteriocin carnobacteriocin B2

Entity

1. type IIA bacteriocin carnobacteriocin B2 (polymer, Thiol state: all disulfide bound), 48 monomers, 4969.448 Da Detail

VNYGNGVSCS KTKCSVNWGQ AFQERYTAGI NSFVSGVASG AGSIGRRP

Formula weight

4969.448 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS9:SG	1:CYS14:SG

Source organism

Carnobacterium maltaromaticum

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 63.9 %, Completeness (bb): 55.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	63.9 % (168 of 263)	63.9 % (168 of 263)
Backbone	55.3 % (57 of 103)	55.3 % (57 of 103)
Sidechain	69.4 % (111 of 160)	69.4 % (111 of 160)
Aromatic	66.7 % (16 of 24)	66.7 % (16 of 24)
Methyl	70.0 % (14 of 20)	70.0 % (14 of 20)

1. type IIA bacteriocin carnobacteriocin B2

VNYGNGVSCS KTKCSVNWGQ AFQERYTAGI NSFVSGVASG AGSIGRRP

Show sample condition

Sample

Temperature 298 K, pH 2.8

#	Name	Isotope labeling	Type	Concentration
1	type IIA bacteriocin carnobacteriocin B2			5 mg
2	TFE-D3			700 uL

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Protein Blocks Logo

Calculated from 20 models in PDB: 1CW5, Strand ID: A Detail

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Coupling constant

8 J values in 1 lists
Temperature 298 K, pH 2.8 Detail

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Temperature 298 K, pH 2.8

List #1 coupling_constants_CARNOBACTERIOCIN

Release date

2000-10-01

Citation

Solution structure of carnobacteriocin B2 and implications for structure-activity relationships among type IIa bacteriocins from lactic acid bacteria
Wang, Y., Henz, M.E., Gallagher, N.L.F., Chai, S., Yan, L.Z., Gibbs, A.C., Stiles, M.E., Wishart, D.S., Vederas, J.C.
Biochemistry (1999), 38, 15438-15447, PubMed 10569926 , DOI: , Abstract

Related entities 1. type IIA bacteriocin carnobacteriocin B2, : 1 : 1 : 1 : 4 entities Detail

Experiments performed 1 experiments Detail