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Solution Structure of Carnobacteriocin B2 and Implications for Structure- Activity Relationships Among Type IIa Bacteriocins from Lactic Acid Bacteria
Authors
Wang, Y., Henz, M.E., Gallagher, N.L.F., Chai, S., Yan, L.Z., Gibbs, A.C., Stiles, M.E., Wishart, D.S., Vederas, J.C.
Assembly
Type IIa bacteriocin leucocin A
Entity
1. Type IIa bacteriocin leucocin A (polymer, Thiol state: all disulfide bound), 37 monomers, 3932.280 Da Detail

KYYGNGVHCT KSGCSVNWGE AFSAGVHRLA NGGNGFW


Formula weight
3932.28 Da
Entity Connection
disulfide 1 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS9:SG1:CYS14:SG

Source organism
Leuconostoc gelidum
Exptl. method
NMR
Refine. method
SIMULATED ANNEALING
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 68.1 %, Completeness (bb): 56.1 % Detail

Polymer type: polypeptide(L)

Total1H
All68.1 % (139 of 204)68.1 % (139 of 204)
Backbone56.1 % (46 of 82)56.1 % (46 of 82)
Sidechain76.2 % (93 of 122)76.2 % (93 of 122)
Aromatic47.1 % (16 of 34)47.1 % (16 of 34)
Methyl100.0 % (12 of 12)100.0 % (12 of 12)

1. Type IIA bacteriocin leucocin A

KYYGNGVHCT KSGCSVNWGE AFSAGVHRLA NGGNGFW

Sample

Temperature 298 K, pH 2.8


#NameIsotope labelingTypeConcentration
1Type IIA bacteriocin leucocin A2 mM
2TFE[U-2H]90 %
3H2O10 %

Protein Blocks Logo
Calculated from 18 models in PDB: 1CW6, Strand ID: A Detail


Release date
1999-12-05
Citation
Genes of the sbo-alb locus of Bacillus subtilis are required for production of the antilisterial bacteriocin subtilosin
Wang, Y., Henz, M.E., Gallagher, N.L.F., Chai, S., Yan, L.Z., Gibbs, A.C., Stiles, M.E., Wishart, D.S., Vederas, J.C.
J. Bacteriol. (1999), 181, 7346-7355, PubMed 10572140 ,
Entries sharing articles Swiss-Prot: 8 entries Detail
  Swiss-Prot: O07623 released on 2003-01-10
    Title SBOA_BACSU Entity Subtilosin-A
  Swiss-Prot: P71005 released on 2003-01-10
    Title ALBG_BACSU Entity Antilisterial bacteriocin subtilosin biosynthesis protein AlbG
  Swiss-Prot: P71006 released on 2003-01-10
    Title ALBF_BACSU Entity Putative zinc protease AlbF
  Swiss-Prot: P71007 released on 2003-01-10
    Title ALBE_BACSU Entity Antilisterial bacteriocin subtilosin biosynthesis protein AlbE
  Swiss-Prot: P71008 released on 2003-01-10
    Title ALBD_BACSU Entity Antilisterial bacteriocin subtilosin biosynthesis protein AlbD
  Swiss-Prot: P71009 released on 2003-01-10
    Title ALBC_BACSU Entity Putative ABC transporter ATP-binding protein AlbC
  Swiss-Prot: P71010 released on 2003-01-10
    Title ALBB_BACSU Entity Antilisterial bacteriocin subtilosin biosynthesis protein AlbB
  Swiss-Prot: P71011 released on 2003-01-10
    Title ALBA_BACSU Entity Antilisterial bacteriocin subtilosin biosynthesis protein AlbA
Related entities 1. Type IIa bacteriocin leucocin A, : 1 : 4 : 2 : 16 entities Detail
Experiments performed 1 experiments Detail
nullKeywords antimicrobial peptide, bacteriocin