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The Second Type II Module From Human Matrix Metalloproteinase 2
Authors
Briknarova, K., Grishaev, A., Banyai, L., Tordai, H., Patthy, L., Llinas, M.
Assembly
The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2
Entity
1. The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2 (polymer, Thiol state: all disulfide bound), 60 monomers, 6766.305 Da Detail

TALFTMGGNA EGQPCKFPFR FQGTSYDSCT TEGRTDGYRW CGTTEDYDRD KKYGFCPETA


Formula weight
6766.305 Da
Entity Connection
disulfide 2 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS15:SG1:CYS41:SG
2disulfidesing1:CYS29:SG1:CYS56:SG

Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
DISTANCE GEOMETRY AND SIMULATED ANNEALING
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 98.0 %, Completeness (bb): 97.3 % Detail

Polymer type: polypeptide(L)

Total1H15N
All98.0 % (400 of 408)98.0 % (340 of 347)98.4 % (60 of 61)
Backbone97.3 % (177 of 182)96.8 % (121 of 125)98.2 % (56 of 57)
Sidechain98.7 % (223 of 226)98.6 % (219 of 222)100.0 % (4 of 4)
Aromatic100.0 % (48 of 48)100.0 % (47 of 47)100.0 % (1 of 1)
Methyl100.0 % (14 of 14)100.0 % (14 of 14)

1. The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2

TALFTMGGNA EGQPCKFPFR FQGTSYDSCT TEGRTDGYRW CGTTEDYDRD KKYGFCPETA

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 5.2 (±0.2)


#NameIsotope labelingTypeConcentration
1The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 21.3 mM
2H2O90 %
3D2O10 %
Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 5.2 (±0.2)


#NameIsotope labelingTypeConcentration
4The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2[U-15N]1 mM
5H2O90 %
6D2O10 %
Sample #3

Pressure 1 atm, Temperature 298 (±1) K, pH 5.2 (±0.2)


#NameIsotope labelingTypeConcentration
7The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2[U-15N]4 mM
8H2O90 %
9D2O10 %

Protein Blocks Logo
Calculated from 50 models in PDB: 1CXW, Strand ID: A Detail


Release date
2000-05-24
Citation
The second type II module from human matrix metalloproteinase 2: structure, function and dynamics
Briknarova, K., Grishaev, A., Banyai, L., Tordai, H., Patthy, L., Llinas, M.
Structure (1999), 7, 1235-1245, PubMed 10545322 , DOI: ,
Related entities 1. The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2, : 1 : 5 : 172 entities Detail
Interaction partners 1. The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2, : 16 interactors Detail
Experiments performed 11 experiments Detail
nullKeywords collagenase, fibronectin type II module, gelatinase, MMP-2