BMRB: 4514

NMR solution structure of complement-like repeat CR3 from the low density lipoprotein receptor-related protein (LRP). Evidence for specific binding to the receptor binding domain of human alpha-2 macroglobulin

Authors

Dolmer, K., Huang, W., Gettins, P.G.W.

Assembly

Third complement-like repeat (CR3) from lipoprotein receptor related protein

Entity

1. lipoprotein receptor related protein (polymer, Thiol state: all disulfide bound), 45 monomers, 4947.310 Da Detail

GSPPQCQPGE FACANSRCIQ ERWKCDGDND CLDNSDEAPA LCHQH

2. CA (non-polymer), 40.078 Da

Total weight

4987.388 Da

Max. entity weight

4947.31 Da

Entity Connection

na 6, disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS6:SG
2	disulfide	sing	1:CYS13:SG	1:CYS31:SG
3	disulfide	sing	1:CYS25:SG	1:CYS42:SG
4	na	sing	2:CA1:CA	1:TRP23:O
5	na	sing	2:CA1:CA	1:ASP26:OD1
6	na	sing	2:CA1:CA	1:ASP28:O
7	na	sing	2:CA1:CA	1:ASP30:OD1
8	na	sing	2:CA1:CA	1:ASP36:OD1
9	na	sing	2:CA1:CA	1:GLU37:OE1

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

MOLECULAR DYNAMICS ENERGY MINIMIZATION

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 66.0 %, Completeness (bb): 76.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	66.0 % (316 of 479)	79.1 % (200 of 253)	42.9 % (76 of 177)	81.6 % (40 of 49)
Backbone	76.0 % (199 of 262)	94.4 % (84 of 89)	56.8 % (75 of 132)	97.6 % (40 of 41)
Sidechain	57.9 % (150 of 259)	70.7 % (116 of 164)	39.1 % (34 of 87)	0.0 % (0 of 8)
Aromatic	0.0 % (0 of 30)	0.0 % (0 of 15)	0.0 % (0 of 14)	0.0 % (0 of 1)
Methyl	65.0 % (13 of 20)	100.0 % (10 of 10)	30.0 % (3 of 10)

1. lipoprotein receptor related protein

GSPPQCQPGE FACANSRCIQ ERWKCDGDND CLDNSDEAPA LCHQH

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	lipoprotein receptor related protein	[U-15N; U-13C]		2 mM
2	sodium acetate	[U-2H]		20 mM
3	sodium chloride			10 mM
4	H2O			90 %
5	D2O			10 %

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LACS Plot; CA

Referencing offset: 0.7 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: 0.7 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: 0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 1D2L, Strand ID: A Detail

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Release date

2001-05-06

Citation

NMR solution structure of complement-like repeat CR3 from the low density lipoprotein receptor-related protein. Evidence for specific binding to the receptor binding domain of human alpha(2)-macroglobulin
Dolmer, K., Huang, W., Gettins, P.G.W.
J. Biol. Chem. (2000), 275, 3264-3269, PubMed 10652313 , Abstract

Related entities 1. lipoprotein receptor related protein, : 1 : 1 : 647 entities Detail

Interaction partners 1. lipoprotein receptor related protein, : 23 interactors Detail

Experiments performed 6 experiments Detail