BMRBj - BMREntryMaster

Found 1 Document (2.345 seconds)

BMRB: 4519

1D7Q

HUMAN TRANSLATION INITIATION FACTOR EIF1A

Authors

Battiste, J.L., Pestova, T.V., Hellen, C.U.T., Wagner, G.

Assembly

Translation initiation factor 1A

Entity

1. Translation initiation factor 1A (polymer, Thiol state: not reported), 157 monomers, 18027.86 Da Detail

MRGSHHHHHH TDPMPKNKGK GGKNRRRGKN ENESEKRELV FKEDGQEYAQ VIKMLGNGRL EAMCFDGVKR LCHIRGKLRK KVWINTSDII LVGLRDYQDN KADVILKYNA DEARSLKAYG ELPEHAKINE TDTFGPGDDD EIQFDDIGDD DEDIDDI

Formula weight

18027.86 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

STRUCTURES WERE CALCULATED USING SIMULATED ANNEALING/MOLECULAR DYNAMICS FROM

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.4 %, Completeness: 78.3 %, Completeness (bb): 85.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.3 % (1433 of 1830)	78.9 % (764 of 968)	76.7 % (533 of 695)	81.4 % (136 of 167)
Backbone	85.2 % (796 of 934)	84.9 % (276 of 325)	85.7 % (391 of 456)	84.3 % (129 of 153)
Sidechain	72.5 % (753 of 1038)	75.9 % (488 of 643)	67.7 % (258 of 381)	50.0 % (7 of 14)
Aromatic	40.5 % (47 of 116)	79.3 % (46 of 58)	0.0 % (0 of 57)	100.0 % (1 of 1)
Methyl	100.0 % (130 of 130)	100.0 % (65 of 65)	100.0 % (65 of 65)

1. Translation initiation factor 1A

MRGSHHHHHH TDPMPKNKGK GGKNRRRGKN ENESEKRELV FKEDGQEYAQ VIKMLGNGRL EAMCFDGVKR LCHIRGKLRK KVWINTSDII LVGLRDYQDN KADVILKYNA DEARSLKAYG ELPEHAKINE TDTFGPGDDD EIQFDDIGDD DEDIDDI

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	Translation initiation factor 1A	[U-13C; U-15N]	0.8 mM
2	phosphate buffer		10 mM
3	NaCl		500 mM
4	DTT		1 mM
5	EDTA		0.1 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
6	Translation initiation factor 1A	[U-15N]	0.5 mM
7	phosphate buffer		10 mM
8	NaCl		500 mM
9	DTT		1 mM
10	EDTA		0.1 mM

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Concentration
11	Translation initiation factor 1A	1.2 mM
12	phosphate buffer	10 mM
13	NaCl	500 mM
14	DTT	1 mM
15	EDTA	0.1 mM

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
16	Translation initiation factor 1A	[U-15N]-Lys	0.4 mM
17	phosphate buffer		10 mM
18	NaCl		500 mM
19	DTT		1 mM
20	EDTA		0.1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹³C	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.31 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹³C	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.31 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹³C	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹³C	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.12 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1D7Q, Strand ID: A, B Detail

Release date

2000-07-11

Citation

The eIF1A solution structure reveals a large RNA-binding surface important for scanning function
Battiste, J.L., Pestova, T.V., Hellen, C.U.T., Wagner, G.
Mol. Cell (2000), 5, 109-119, PubMed 10678173 , DOI: , Abstract

Related entities 1. Translation initiation factor 1A, : 1 : 24 : 53 entities Detail

Interaction partners 1. Translation initiation factor 1A, : 18 interactors Detail

More details for 18 interactors identified by 8 citations

Experiments performed 3 experiments Detail

1 3D 13C-separated NOESY

Instrument

Model Varian Unity (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	Translation initiation factor 1A	[U-13C; U-15N]	0.8 mM
2	phosphate buffer		10 mM
3	NaCl		500 mM
4	DTT		1 mM
5	EDTA		0.1 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
6	Translation initiation factor 1A	[U-15N]	0.5 mM
7	phosphate buffer		10 mM
8	NaCl		500 mM
9	DTT		1 mM
10	EDTA		0.1 mM

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Concentration
11	Translation initiation factor 1A	1.2 mM
12	phosphate buffer	10 mM
13	NaCl	500 mM
14	DTT	1 mM
15	EDTA	0.1 mM

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
16	Translation initiation factor 1A	[U-15N]-Lys	0.4 mM
17	phosphate buffer		10 mM
18	NaCl		500 mM
19	DTT		1 mM
20	EDTA		0.1 mM

2 3D 15N-separated NOESY

Instrument

Model Varian Unity (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	Translation initiation factor 1A	[U-13C; U-15N]	0.8 mM
2	phosphate buffer		10 mM
3	NaCl		500 mM
4	DTT		1 mM
5	EDTA		0.1 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
6	Translation initiation factor 1A	[U-15N]	0.5 mM
7	phosphate buffer		10 mM
8	NaCl		500 mM
9	DTT		1 mM
10	EDTA		0.1 mM

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Concentration
11	Translation initiation factor 1A	1.2 mM
12	phosphate buffer	10 mM
13	NaCl	500 mM
14	DTT	1 mM
15	EDTA	0.1 mM

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
16	Translation initiation factor 1A	[U-15N]-Lys	0.4 mM
17	phosphate buffer		10 mM
18	NaCl		500 mM
19	DTT		1 mM
20	EDTA		0.1 mM

3 2D NOESY

Instrument

Model Varian Unity (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	Translation initiation factor 1A	[U-13C; U-15N]	0.8 mM
2	phosphate buffer		10 mM
3	NaCl		500 mM
4	DTT		1 mM
5	EDTA		0.1 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
6	Translation initiation factor 1A	[U-15N]	0.5 mM
7	phosphate buffer		10 mM
8	NaCl		500 mM
9	DTT		1 mM
10	EDTA		0.1 mM

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Concentration
11	Translation initiation factor 1A	1.2 mM
12	phosphate buffer	10 mM
13	NaCl	500 mM
14	DTT	1 mM
15	EDTA	0.1 mM

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
16	Translation initiation factor 1A	[U-15N]-Lys	0.4 mM
17	phosphate buffer		10 mM
18	NaCl		500 mM
19	DTT		1 mM
20	EDTA		0.1 mM

nullKeywords OB-fold, beta-barrel, RNA-binding protein

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Found 1 Document (2.345 seconds)

BMRB: 4519

Sample #1

Sample #2

Sample #3

Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Translation initiation factor 1A, : 1 : 24 : 53 entities Detail

Interaction partners 1. Translation initiation factor 1A, : 18 interactors Detail

Experiments performed 3 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4