BMRB: 4524

Solution Structure of a Type-I Dockerin Domain, a Novel Prokaryotic, Extracellular Calcium-Binding Domain

Authors

Lytle, B.L., Volkman, B.F., Westler, W.M., Heckman, M.P., Wu, J.H.D.

Assembly

Endoglucanase SS dockerin domain

Entity

1. DOCKERIN DOMAIN (polymer, Thiol state: not present), 71 monomers, 7848.685 Da Detail

MSTKLYGDVN DDGKVNSTDA VALKRYVLRS GISINTDNAD LNEDGRVNST DLGILKRYIL KEIDTLPYKN G

2. CA (non-polymer), 40.078 Da

Total weight

7888.763 Da

Max. entity weight

7848.685 Da

Source organism

Acetivibrio thermocellus

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.7 %, Completeness: 86.2 %, Completeness (bb): 88.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	86.2 % (424 of 492)	85.5 % (355 of 415)	89.6 % (69 of 77)
Backbone	88.5 % (192 of 217)	87.8 % (129 of 147)	90.0 % (63 of 70)
Sidechain	84.4 % (232 of 275)	84.3 % (226 of 268)	85.7 % (6 of 7)
Aromatic	100.0 % (16 of 16)	100.0 % (16 of 16)
Methyl	97.7 % (43 of 44)	97.7 % (43 of 44)

1. DOCKERIN DOMAIN

MSTKLYGDVN DDGKVNSTDA VALKRYVLRS GISINTDNAD LNEDGRVNST DLGILKRYIL KEIDTLPYKN G

Show sample condition

Sample

Pressure 1 atm, Temperature 328 (±1) K, pH 6.0 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	DOCKERIN DOMAIN			0.8 mM
2	potassium chloride			100 mM
3	calcium chloride			20 mM
4	D2O			10 %
5	H2O			90 %

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Protein Blocks Logo

Calculated from 1 models in PDB: 1DAQ, Strand ID: A Detail

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Release date

2002-03-28

Citation

Solution structure of a type I dockerin domain, a novel prokaryotic, extracellular calcium-binding domain
Lytle, B.L., Volkman, B.F., Westler, W.M., Heckman, M.P., Wu, J.H.D.
J. Mol. Biol. (2001), 307, 745-753, PubMed 11273698 , DOI 10.1006/jmbi.2001.4522 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. DOCKERIN DOMAIN, : 1 : 1 : 2 : 46 entities Detail

Experiments performed 3 experiments Detail