BMRB: 4536

Structural basis for uracil DNA glycosylase interaction with uracil: NMR study

Authors

Ghosh, M., Kumar1, V., Varshney, U., Chary, K.V.R.

Assembly

5'-D(*AP*GP*GP*AP*TP*CP*CP*TP*UP*TP*TP*GP*GP*AP*TP*CP*CP*T)-3'

Entity

1. 5'-D(*AP*GP*GP*AP*TP*CP*CP*TP*UP*TP*TP*GP*GP*AP*TP*CP*CP*T)-3' (polymer, Thiol state: not present), 22 monomers, 6702.315 Da Detail

CTAGAGGATC CTXTTGGATC CT

Formula weight

6702.315 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 84.5 %, Completeness (bb): 84.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polydeoxyribonucleotide

	Total	1H
All	84.5 % (174 of 206)	84.5 % (174 of 206)
Suger, PO4	84.4 % (124 of 147)	84.4 % (124 of 147)
Nucleobase	84.7 % (50 of 59)	84.7 % (50 of 59)
Aromatic	83.3 % (35 of 42)	83.3 % (35 of 42)
Methyl	100.0 % (7 of 7)	100.0 % (7 of 7)

1. URACIL CONTAINING HAIRPIN DNA

CTAGAGGATC CTXTTGGATC CT

Show sample condition

Sample

Pressure 1 atm, Temperature 305 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	URACIL CONTAINING HAIRPIN DNA			5 mM
2	D2O			99 %

Hide sample codition

Release date

2000-08-31

Citation

Structural basis for uracil DNA glycosylase interaction with uracil: NMR study
Ghosh, M., Kumar1, V., Varshney, U., Chary, K.V.R.
Nucleic Acids Res. (2000), 28, 1906-1912, PubMed 10756190 , DOI: , Abstract

Experiments performed 2 experiments Detail