BMRB: 4542

Solution structure of a uracil containing hairpin DNA

Authors

Ghosh, M., Kumar, N.V., Varshney, U., Chary, K.V.R.

Assembly

5'-D(*AP*GP*GP*AP*TP*CP*CP*TP*TP*TP*UP*GP*GP*AP*TP*CP*CP*T)-3'

Entity

1. 5'-D(*AP*GP*GP*AP*TP*CP*CP*TP*TP*TP*UP*GP*GP*AP*TP*CP*CP*T)-3' (polymer, Thiol state: not present), 22 monomers, 6782.295 Da Detail

CTAGAGGATC CTTTXGGATC CT

Formula weight

6782.295 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 88.8 %, Completeness (bb): 91.2 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	88.8 % (183 of 206)	88.8 % (183 of 206)
Suger, PO4	91.2 % (134 of 147)	91.2 % (134 of 147)
Nucleobase	83.1 % (49 of 59)	83.1 % (49 of 59)
Aromatic	81.0 % (34 of 42)	81.0 % (34 of 42)
Methyl	100.0 % (7 of 7)	100.0 % (7 of 7)

1. 5'-D(*AP*GP*GP*AP*TP*CP*CP*TP*TP*TP*UP*GP*GP*AP*TP*CP*CP*T)-3'

CTAGAGGATC CTTTXGGATC CT

Show sample condition

Sample

Pressure 1 atm, Temperature 305 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	5'-D(*AP*GP*GP*AP*TP*CP*CP*TP*TP*TP*UP*GP*GP*AP*TP*CP*CP*T)-3'			5 mM
2	D2O			99 %

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Release date

2000-12-05

Citation

Structural characterisation of a uracil containing hairpin DNA by NMR and molecular dynamics
Ghosh, M., Kumar, N.V., Varshney, U., Chary, K.V.R.
Nucleic Acids Res. (1999), 27, 3938-3944, PubMed 10481034 , Abstract

Experiments performed 2 experiments Detail