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BMRB: 4547


4547
1C2Q
Solution structure of a DNA.RNA hybrid containing an alpha-anomeric thymidine and polarity reversals: d(ATGG-3'-3'-(alpha-T)-5'-5'-GCTC).r(gagcaccau)
Authors
Germann, M.W., Aramini, J.M.
Assembly
DNA/RNA
Entity
1. 5'-D(ATGG-3'-3'-(ALPHA-T)-5'-5'-GCTC)-3' (polymer, Thiol state: not present), 9 monomers, 2809.781 Da Detail

ATGGXGCTC


2. RNA (polymer, Thiol state: not present), 9 monomers, 2917.755 Da Detail

GAGCACCAU


Total weight
5727.536 Da
Max. entity weight
2917.755 Da
Exptl. method
NMR
Data set
assigned_chemical_shifts, coupling_constants
Chem. Shift Complete1
Sequence coverage: 44.4 %, Completeness: 48.3 %, Completeness (bb): 49.6 % Detail
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Polymer type: polydeoxyribonucleotide polyribonucleotide

Total1H31P
All48.3 % (84 of 174)49.0 % (77 of 157)41.2 % (7 of 17)
Suger, PO449.6 % (63 of 127)50.9 % (56 of 110)41.2 % (7 of 17)
Nucleobase44.7 % (21 of 47)44.7 % (21 of 47)
Aromatic42.9 % (15 of 35)42.9 % (15 of 35)
Methyl100.0 % (2 of 2)100.0 % (2 of 2)

1. 5'-D(ATGG-3'-3'-(ALPHA-T)-5'-5'-GCTC)-3'

ATGGXGCTC

2. RNA

GAGCACCAU

Show sample condition
Sample

Temperature 298 K, pH 6.6


#NameIsotope labelingTypeConcentration
15'-D(ATGG-3'-3'-(ALPHA-T)-5'-5'-GCTC)-3'0.89 mM
2RNA0.89 mM
3NaCl50 mM
4NaPhosphate10 mM
5EDTA0.5 mM
6D2O0.0 ~ 0.0 %

Chem. Shift Complete2
Sequence coverage: 50.0 %, Completeness: 48.0 %, Completeness (bb): 48.4 % Detail
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Polymer type: polydeoxyribonucleotide polyribonucleotide

Total1H31P
All48.0 % (167 of 348)48.4 % (152 of 314)44.1 % (15 of 34)
Suger, PO448.4 % (123 of 254)49.1 % (108 of 220)44.1 % (15 of 34)
Nucleobase46.8 % (44 of 94)46.8 % (44 of 94)
Aromatic45.7 % (32 of 70)45.7 % (32 of 70)
Methyl50.0 % (2 of 4)50.0 % (2 of 4)

1. 5'-D(ATGG-3'-3'-(ALPHA-T)-5'-5'-GCTC)-3'

ATGGXGCTC

2. RNA

GAGCACCAU

Show sample condition
Sample

Temperature 298 K, pH 6.6


#NameIsotope labelingTypeConcentration
15'-D(ATGG-3'-3'-(ALPHA-T)-5'-5'-GCTC)-3'0.89 mM
2RNA0.89 mM
3NaCl50 mM
4NaPhosphate10 mM
5EDTA0.5 mM
6D2O0.0 ~ 0.0 %

Coupling constant
36 J values in 1 lists
Field strength (1H) 600 MHz, Temperature 298 K, pH 6.6 Detail
Release date
2000-12-05
Citation
Solution structure of a DNA.RNA hybrid containing an alpha-anomeric thymidine and polarity reversals: d(ATGG-3'-3'-alphaT-5'-5'-GCTC). r(gagcaccau)
Germann, M.W., Aramini, J.M.
Biochemistry (1999), 38, 15448-15458, PubMed 10569927 ,
Experiments performed 3 experiments Detail
nullKeywords 3'-3', 5'-5' PHOSPHODIESTER LINKAGES, ALPHAT-ANOMERIC THYMIDINE, DNA/RNA HYBRID