Assignment of 1H, 13C and 15N Resonances of the I-domain of Human Leukocyte Function Associated Antigen-1
MASKGNVDLV FLFDGSMSLQ PDEFQKILDF MKDVMKKLSN TSYQFAAVQF STSYKTEFDF SDYVKWKDPD ALLKHVKHML LLTNTFGAIN YVATEVFREE LGARPDATKV LIIITDGEAT DSGNIDAAKD IIRYIIGIGK HFQTKESQET LHKFASKPAS EFVKILDTFE KLKDLFTELQ KKIYVIEGM
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.3 % (2065 of 2288) | 97.7 % (1157 of 1184) | 79.4 % (719 of 906) | 95.5 % (189 of 198) |
Backbone | 84.3 % (949 of 1126) | 98.2 % (376 of 383) | 70.4 % (393 of 558) | 97.3 % (180 of 185) |
Sidechain | 96.3 % (1293 of 1342) | 97.5 % (781 of 801) | 95.3 % (503 of 528) | 69.2 % (9 of 13) |
Aromatic | 93.4 % (211 of 226) | 99.1 % (112 of 113) | 87.5 % (98 of 112) | 100.0 % (1 of 1) |
Methyl | 99.1 % (218 of 220) | 99.1 % (109 of 110) | 99.1 % (109 of 110) |
1. Leukocyte Function Associated Antigen-1 Inserted-Domain
MASKGNVDLV FLFDGSMSLQ PDEFQKILDF MKDVMKKLSN TSYQFAAVQF STSYKTEFDF SDYVKWKDPD ALLKHVKHML LLTNTFGAIN YVATEVFREE LGARPDATKV LIIITDGEAT DSGNIDAAKD IIRYIIGIGK HFQTKESQET LHKFASKPAS EFVKILDTFE KLKDLFTELQ KKIYVIEGMTemperature 295 (±1) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Leukocyte Function Associated Antigen-1 Inserted-Domain | [U-13C; U-15N] | 1.2 mM | |
2 | NaH2PO4 | 10 mM | ||
3 | MgSO4 | 10 mM | ||
4 | NaCl | 150 mM | ||
5 | NaN3 | 0.05 % | ||
6 | 2H2O | 5 % |
Temperature 295 (±1) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Leukocyte Function Associated Antigen-1 Inserted-Domain | [U-15N] | 1.2 mM |