BMRB: 4557

Structure of the active domain of the herpes simplex virus protein ICP47 in water/sodium dodecyl sulfate solution determined by nuclear magnetic resonance spectroscop

Authors

Pfaender, R., Neumann, L., Zweckstetter, M., Seger, C., Holak, T.A., Tampe, R.

Assembly

herpes simplex virus protein ICP47

Entity

1. herpes simplex virus protein ICP47 (polymer, Thiol state: not present), 33 monomers, 3884.319 Da Detail

SWALEMADTF LDNMRVGPRT YADVRDEINK RGR

Formula weight

3884.319 Da

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.9 %, Completeness: 76.1 %, Completeness (bb): 95.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	76.1 % (153 of 201)	76.1 % (153 of 201)
Backbone	95.5 % (64 of 67)	95.5 % (64 of 67)
Sidechain	66.4 % (89 of 134)	66.4 % (89 of 134)
Aromatic	0.0 % (0 of 15)	0.0 % (0 of 15)
Methyl	86.7 % (13 of 15)	86.7 % (13 of 15)

1. herpes simplex virus protein ICP47

SWALEMADTF LDNMRVGPRT YADVRDEINK RGR

Show sample condition

Sample #1

Temperature 307 (±1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	herpes simplex virus protein ICP47			4.7 mM
2	SDS	[U-2H]		560 mM
3	H2O			90 %
4	D2O			10 %

Sample #2

Temperature 307 (±1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	herpes simplex virus protein ICP47			4.7 mM
6	SDS	[U-2H]		560 mM
7	D2O			100 %

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Protein Blocks Logo

Calculated from 19 models in PDB: 1QLO, Strand ID: A Detail

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Release date

2001-08-05

Citation

The structure of the active domain of the herpes simplex virus protein ICP47 in water/sodium dodecyl sulfate solution determined by nuclear magnetic resonance spectroscop
Pfaender, R., Neumann, L., Zweckstetter, M., Seger, C., Holak, T.A., Tampe, R.
Biochemistry (1999), 38, 13692-13698

Related entities 1. herpes simplex virus protein ICP47, : 1 : 1 : 1 : 6 entities Detail

Interaction partners 1. herpes simplex virus protein ICP47, : 2 interactors Detail

Experiments performed 3 experiments Detail