BMRB: 4570

NMR Study of Sso7d Mutant (F31A) Minimized Average Structure

Authors

CONSONNI, R., SANTOMO, L., ZETTA, L.

Assembly

SSO7D

Entity

1. SSO7D (polymer, Thiol state: not present), 62 monomers, 6943.006 Da Detail

ATVKFKYKGE EKQVDISKIK KVWRVGKMIS ATYDEGGGKT GRGAVSEKDA PKELLQMLEK QK

Formula weight

6943.006 Da

Source organism

Saccharolobus solfataricus

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 66.8 %, Completeness (bb): 97.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	66.8 % (267 of 400)	66.8 % (267 of 400)
Backbone	97.7 % (127 of 130)	97.7 % (127 of 130)
Sidechain	51.9 % (140 of 270)	51.9 % (140 of 270)
Aromatic	63.2 % (12 of 19)	63.2 % (12 of 19)
Methyl	75.9 % (22 of 29)	75.9 % (22 of 29)

1. SSO7D

ATVKFKYKGE EKQVDISKIK KVWRVGKMIS ATYDEGGGKT GRGAVSEKDA PKELLQMLEK QK

Show sample condition

Sample

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	SSO7D			2.0 mM

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Calculated from 1 models in PDB: 1B4O, Strand ID: A Detail

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Release date

2001-01-13

Citation

A single-point mutation in the extreme heat- and pressure-resistant sso7d protein from sulfolobus solfataricus leads to a major rearrangement of the hydrophobic core
Consonni, R., Santomo, L., Fusi, P., Tortora, P., Zetta, L.
Biochemistry (1999), 38, 12709-12717, PubMed 10504241 , DOI: , Abstract

Related entities 1. SSO7D, : 1 : 6 : 59 entities Detail

Experiments performed 3 experiments Detail