BMRB: 4574

CIDE-N Domain of Human CIDE-B

Authors

Wagner, G., Lugovskoy, A., Zhou, P., Chou, J., McCarty, J., Li, P.

Assembly

CIDE-N DOMAIN OF HUMAN CIDE-B

Entity

1. CIDE-N DOMAIN OF HUMAN CIDE-B (polymer, Thiol state: all free), 122 monomers, 13729.38 Da Detail

MEYLSALNPS DLLRSVSNIS SEFGRRVWTS APPPQRPFRV CDHKRTIRKG LTAATRQELL AKALETLLLN GVLTLVLEED GTAVDSEDFF QLLEDDTCLM VLQSGQSWSP TRSGVLHHHH HH

Formula weight

13729.38 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY SIMULATED ANNEALING TORSION ANGLE DYNAMICS RELAXATION DATA

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.6 %, Completeness: 78.3 %, Completeness (bb): 91.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	78.3 % (1099 of 1403)	82.3 % (597 of 725)	71.2 % (393 of 552)	86.5 % (109 of 126)
Backbone	91.0 % (655 of 720)	91.0 % (222 of 244)	90.6 % (326 of 360)	92.2 % (107 of 116)
Sidechain	68.3 % (546 of 799)	78.0 % (375 of 481)	54.9 % (169 of 308)	20.0 % (2 of 10)
Aromatic	27.0 % (27 of 100)	50.0 % (25 of 50)	0.0 % (0 of 48)	100.0 % (2 of 2)
Methyl	96.6 % (143 of 148)	98.6 % (73 of 74)	94.6 % (70 of 74)

1. Human Cell Death-Inducing Effector B

MEYLSALNPS DLLRSVSNIS SEFGRRVWTS APPPQRPFRV CDHKRTIRKG LTAATRQELL AKALETLLLN GVLTLVLEED GTAVDSEDFF QLLEDDTCLM VLQSGQSWSP TRSGVLHHHH HH

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Human Cell Death-Inducing Effector B	[U-13C; U-15N]		0.5 mM

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LACS Plot; CA

Referencing offset: 0.42 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 na	null	indirect	0.0
13C	methyl iodine	methyl protons	0.0 na	null	indirect	0.0
15N	15NHNO3	N	0.0 na	null	direct	0.0

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LACS Plot; CB

Referencing offset: 0.42 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 na	null	indirect	0.0
13C	methyl iodine	methyl protons	0.0 na	null	indirect	0.0
15N	15NHNO3	N	0.0 na	null	direct	0.0

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LACS Plot; HA

Referencing offset: 0.11 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 na	null	indirect	0.0
13C	methyl iodine	methyl protons	0.0 na	null	indirect	0.0
15N	15NHNO3	N	0.0 na	null	direct	0.0

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LACS Plot; CO

Referencing offset: 2.59 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 na	null	indirect	0.0
13C	methyl iodine	methyl protons	0.0 na	null	indirect	0.0
15N	15NHNO3	N	0.0 na	null	direct	0.0

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Protein Blocks Logo

Calculated from 15 models in PDB: 1D4B, Strand ID: A Detail

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Release date

2001-03-01

Citation

Solution Structure of the CIDE-N Domain of CIDE-B and a Model for CIDE-N/CIDE-N Interactions in the DNA Fragmenttion Pathway of Apoptosis
Lugovskoy, A.A., Zhou, P., Chou, J.J., McCarty, J.S., Li, P., Wagner, G.
Cell (1999), 99, 747-755, PubMed , DOI:

Related entities 1. CIDE-N DOMAIN OF HUMAN CIDE-B, : 1 : 18 entities Detail

Interaction partners 1. CIDE-N DOMAIN OF HUMAN CIDE-B, : 2 interactors Detail