BMRB: 4581

Site-site Communication in the EF-hand Ca2+ Binding Protein Calbindin D9k

Authors

Maler, L., Blankenship, J., Rance, M., Chazin, W.J.

Assembly

CALBINDIN D9K

Entity

1. CALBINDIN D9K (polymer, Thiol state: not present), 75 monomers, 8491.528 Da Detail

KSPEELKGIF EKYAAKEGDP NQLSKEELKL LLQTEFPSLL KGMSTLDELF EELDKAGDGE VSFEEFQVLV KKISQ

Formula weight

8491.528 Da

Source organism

Bos taurus

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY FOLLOWED BY SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.9 %, Completeness (bb): 99.1 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	15N
All	85.9 % (480 of 559)	84.9 % (409 of 482)	92.2 % (71 of 77)
Backbone	99.1 % (222 of 224)	100.0 % (152 of 152)	97.2 % (70 of 72)
Sidechain	77.0 % (258 of 335)	77.9 % (257 of 330)	20.0 % (1 of 5)
Aromatic	58.6 % (17 of 29)	58.6 % (17 of 29)
Methyl	100.0 % (39 of 39)	100.0 % (39 of 39)

1. CALBINDIN D9K

KSPEELKGIF EKYAAKEGDP NQLSKEELKL LLQTEFPSLL KGMSTLDELF EELDKAGDGE VSFEEFQVLV KKISQ

Show sample condition

Sample #1

Pressure 1 atm, Temperature 300 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	CALBINDIN D9K			4 mM

Sample #2

Pressure 1 atm, Temperature 300 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	CALBINDIN D9K	[U-15N]		4 mM

Hide sample codition

Protein Blocks Logo

Calculated from 24 models in PDB: 1D1O, Strand ID: A Detail

---

Release date

2000-12-17

Citation 1

Site-site communication in the EF-hand Ca2+-binding protein calbindin D9k
Maler, L., Blankenship, J., Rance, M., Chazin, W.J.
Nat. Struct. Biol. (2000), 7, 245-250, PubMed 10700285 , DOI 10.1038/73369 , Abstract

Show more 1 citaion

Citation 2

Characterization of the N-terminal half-saturated state of calbindin D9k: NMR studies of the N56A mutant
Wimberly, B., Thulin, E., Chazin, W.J.
Protein Sci. (1995), 4, 1045-1055, PubMed 7549869 , DOI 10.1002/pro.5560040603 , Abstract

Hide non-primary 1 citaion

Related entities 1. CALBINDIN D9K, : 1 : 17 : 262 entities Detail

Experiments performed 3 experiments Detail