BMRB: 4583

The C-terminal Domain of the RNA Polymerase Alpha Subunit from Thermus Thermophilus

Authors

Wada, T., Yamazaki, T., Kyogoku, Y.

Assembly

RNA POLYMERASE ALPHA SUBUNIT (2.7.7.-)

Entity

1. RNA POLYMERASE ALPHA SUBUNIT (2.7.7.-) (polymer, Thiol state: not present), 82 monomers, 9100.197 Da Detail

AAPEEAKEPE APPEQEEELD LPLEELGLST RVLHSLKEEG IESVRALLAL NLKDLKNIPG IGERSLEEIK EALEKKGFTL KE

Formula weight

9100.197 Da

Source organism

Thermus thermophilus

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY, SIMULATED ANNEALING, MOLECULAR DYNAMICS

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 76.4 %, Completeness (bb): 84.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	76.4 % (734 of 961)	76.8 % (391 of 509)	71.3 % (266 of 373)	97.5 % (77 of 79)
Backbone	84.4 % (405 of 480)	97.5 % (159 of 163)	70.5 % (170 of 241)	100.0 % (76 of 76)
Sidechain	72.2 % (403 of 558)	67.1 % (232 of 346)	81.3 % (170 of 209)	33.3 % (1 of 3)
Aromatic	0.0 % (0 of 14)	0.0 % (0 of 7)	0.0 % (0 of 7)
Methyl	89.2 % (91 of 102)	90.2 % (46 of 51)	88.2 % (45 of 51)

1. RNA POLYMERASE ALPHA SUBUNIT

AAPEEAKEPE APPEQEEELD LPLEELGLST RVLHSLKEEG IESVRALLAL NLKDLKNIPG IGERSLEEIK EALEKKGFTL KE

Show sample condition

Sample

Pressure 1 atm, Temperature 310 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	RNA POLYMERASE ALPHA SUBUNIT	[U-13C; U-15N]		2 mM
2	phosphate			20 mM
3	KCl			30 mM
4	H2O			90 %
5	D2O			10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.2 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.2 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 1 models in PDB: 1DOQ, Strand ID: A Detail

---

Coupling constant

69 J values in 1 lists
Pressure 1 atm, Temperature 310 K, pH 6.0 Detail

---

Pressure 1 atm, Temperature 310 K, pH 6.0

List #1 coupling_constants

Release date

2000-12-18

Citation

The structure and the characteristic DNA binding property of the C-terminal domain of the RNA polymerase alpha subunit from Thermus thermophilus
Wada, T., Yamazaki, T., Kyogoku, Y.
J. Biol. Chem. (2000), 275, 16057-16063, PubMed 10821859 , Abstract

Related entities 1. RNA POLYMERASE ALPHA SUBUNIT (2.7.7.-), : 1 : 45 : 2 : 81 entities Detail

Interaction partners 1. RNA POLYMERASE ALPHA SUBUNIT (2.7.7.-), : 2 interactors Detail