BMRB: 4585

Solution Structure of BmP02, a new Potassium channel Blocker from the Venom of the Chinese Scorpion Buthus martensi Karsch

Authors

Xu, Y.Q., Wu, J.H., Pei, J.M., Shi, Y.Y., Ji, Y.H., Tong, Q.C.

Assembly

BMP02 NEUROTOXIN

Entity

1. BMP02 NEUROTOXIN (polymer, Thiol state: all disulfide bound), 28 monomers, 2955.391 Da Detail

VGCEECPMHC KGKNAKPTCD DGVCNCNV

Formula weight

2955.391 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS3:SG	1:CYS19:SG
2	disulfide	sing	1:CYS6:SG	1:CYS24:SG
3	disulfide	sing	1:CYS10:SG	1:CYS26:SG

Source organism

Mesobuthus martensii

Exptl. method

NMR

Refine. method

simulated annealing and ENERGY MINIMIZATION

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.0 %, Completeness (bb): 98.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	94.0 % (141 of 150)	94.0 % (141 of 150)
Backbone	98.2 % (56 of 57)	98.2 % (56 of 57)
Sidechain	91.4 % (85 of 93)	91.4 % (85 of 93)
Aromatic	100.0 % (2 of 2)	100.0 % (2 of 2)
Methyl	100.0 % (8 of 8)	100.0 % (8 of 8)

1. BMP02 NEUROTOXIN

VGCEECPMHC KGKNAKPTCD DGVCNCNV

Show sample condition

Sample

Pressure 1 (±0.01) atm, Temperature 300 (±1) K, pH 4.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	BMP02 NEUROTOXIN			3.0 mM
2	H2O			90 %
3	D2O			10 %

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Protein Blocks Logo

Calculated from 25 models in PDB: 1DU9, Strand ID: A Detail

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Release date

2000-12-04

Citation 1

Solution structure of BmP02, a new potassium channel blocker from the venom of the Chinese scorpion Buthus martensi Karsch
Xu, Y.Q., Wu, J.H., Pei, J.M., Shi, Y.Y., Ji, Y.H., Tong, Q.C.
Biochemistry (2000), 39, 13669-13675, PubMed 11076505 , DOI: , Abstract

Show more 1 citaion

Citation 2

Crystallography & NMR system: A new software suite for macromolecular structure determination
Brunger, A.T., Adams, P.D., Clore, G.M., DeLano, W.L., Gros, P., Grosse-Kunstleve, R.W., Jiang, J.S., Kuszewski, J., Nilges, M., Pannu, N.S., Read, R.J., Rice, L.M., Simonson, T., Warren, G.L.
Acta Crystallogr. D. Biol. Crystallogr. (1998), 54, 905-921, PubMed 9757107 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. BMP02 NEUROTOXIN, : 1 : 1 : 1 : 6 : 19 entities Detail

Experiments performed 3 experiments Detail