BMRBj - BMREntryMaster

	Total	¹H	¹⁵N
All	75.8 % (304 of 401)	75.4 % (252 of 334)	77.6 % (52 of 67)
Backbone	71.7 % (129 of 180)	71.0 % (88 of 124)	73.2 % (41 of 56)
Sidechain	79.2 % (175 of 221)	78.1 % (164 of 210)	100.0 % (11 of 11)
Aromatic	77.1 % (27 of 35)	75.0 % (24 of 32)	100.0 % (3 of 3)
Methyl	92.9 % (26 of 28)	92.9 % (26 of 28)

1. CHITINASE A1

MASMTGGGGM GRGSAWQVNT AYTAGQLVTY NGKTYKCLQP HTSLAGWEPS NVPALWQLQ

Show sample condition

Sample

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CHITINASE A1	[U-15N]	2.0 mM
2	KH2PO4/K2HPO4		10 mM
3	dithiothreitol	[U-2H]	10 mM
4	H2O		90 %
5	D2O		10 %

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Calculated from 30 models in PDB: 1ED7, Strand ID: A Detail

Coupling constant

31 J values in 1 lists
Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1) Detail

Release date

2000-11-28

Citation

Solution structure of the chitin-binding domain of Bacillus circulans WL-12 chitinase A1
Ikegami, T., Okada, T., Hashimoto, M., Seino, S., Watanabe, T., Shirakawa, M.
J. Biol. Chem. (2000), 275, 13654-13661, PubMed 10788483 , DOI 10.2210/pdb1ed7/pdb , Abstract

Related entities 1. ChBD-ChiA1, : 14 entities Detail

Experiments performed 5 experiments Detail

1 2D NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CHITINASE A1	[U-15N]	2.0 mM
2	KH2PO4/K2HPO4		10 mM
3	dithiothreitol	[U-2H]	10 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
6	CHITINASE A1	[U-15N]	1.2 mM
7	KH2PO4/K2HPO4		100 mM
8	dithiothreitol	[U-2H]	10 mM
9	D2O		99.8 %

2 3D 15N-separated NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CHITINASE A1	[U-15N]	2.0 mM
2	KH2PO4/K2HPO4		10 mM
3	dithiothreitol	[U-2H]	10 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
6	CHITINASE A1	[U-15N]	1.2 mM
7	KH2PO4/K2HPO4		100 mM
8	dithiothreitol	[U-2H]	10 mM
9	D2O		99.8 %

3 HMQC-J

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CHITINASE A1	[U-15N]	2.0 mM
2	KH2PO4/K2HPO4		10 mM
3	dithiothreitol	[U-2H]	10 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
6	CHITINASE A1	[U-15N]	1.2 mM
7	KH2PO4/K2HPO4		100 mM
8	dithiothreitol	[U-2H]	10 mM
9	D2O		99.8 %

4 3D (H)NNH-TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CHITINASE A1	[U-15N]	2.0 mM
2	KH2PO4/K2HPO4		10 mM
3	dithiothreitol	[U-2H]	10 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
6	CHITINASE A1	[U-15N]	1.2 mM
7	KH2PO4/K2HPO4		100 mM
8	dithiothreitol	[U-2H]	10 mM
9	D2O		99.8 %

5 2D H(NN)H-TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CHITINASE A1	[U-15N]	2.0 mM
2	KH2PO4/K2HPO4		10 mM
3	dithiothreitol	[U-2H]	10 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
6	CHITINASE A1	[U-15N]	1.2 mM
7	KH2PO4/K2HPO4		100 mM
8	dithiothreitol	[U-2H]	10 mM
9	D2O		99.8 %

nullKeywords twisted beta-sandwich, CBD, cellulose, ChBD, chitin binding domain, ChiA1, chitinase A1, NMR

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BMRB: 4588

Sample

Related entities 1. ChBD-ChiA1, : 14 entities Detail

Experiments performed 5 experiments Detail

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