BMRBj - BMREntryMaster

	Total	¹H	¹⁵N
All	75.8 % (304 of 401)	75.4 % (252 of 334)	77.6 % (52 of 67)
Backbone	71.7 % (129 of 180)	71.0 % (88 of 124)	73.2 % (41 of 56)
Sidechain	79.2 % (175 of 221)	78.1 % (164 of 210)	100.0 % (11 of 11)
Aromatic	77.1 % (27 of 35)	75.0 % (24 of 32)	100.0 % (3 of 3)
Methyl	92.9 % (26 of 28)	92.9 % (26 of 28)

1. CHITINASE A1

MASMTGGGGM GRGSAWQVNT AYTAGQLVTY NGKTYKCLQP HTSLAGWEPS NVPALWQLQ

Show sample condition

Sample

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CHITINASE A1	[U-15N]	2.0 mM
2	KH2PO4/K2HPO4		10 mM
3	dithiothreitol	[U-2H]	10 mM
4	H2O		90 %
5	D2O		10 %

Protein Blocks Logo

Calculated from 30 models in PDB: 1ED7, Strand ID: A Detail

Coupling constant

31 J values in 1 lists
Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1) Detail

Release date

2000-11-28

Citation

Solution structure of the chitin-binding domain of Bacillus circulans WL-12 chitinase A1
Ikegami, T., Okada, T., Hashimoto, M., Seino, S., Watanabe, T., Shirakawa, M.
J. Biol. Chem. (2000), 275, 13654-13661, PubMed 10788483 , DOI 10.2210/pdb1ed7/pdb , Abstract

Related entities 1. ChBD-ChiA1, : 14 entities Detail

Experiments performed 5 experiments Detail

1 2D NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CHITINASE A1	[U-15N]	2.0 mM
2	KH2PO4/K2HPO4		10 mM
3	dithiothreitol	[U-2H]	10 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
6	CHITINASE A1	[U-15N]	1.2 mM
7	KH2PO4/K2HPO4		100 mM
8	dithiothreitol	[U-2H]	10 mM
9	D2O		99.8 %

2 3D 15N-separated NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CHITINASE A1	[U-15N]	2.0 mM
2	KH2PO4/K2HPO4		10 mM
3	dithiothreitol	[U-2H]	10 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
6	CHITINASE A1	[U-15N]	1.2 mM
7	KH2PO4/K2HPO4		100 mM
8	dithiothreitol	[U-2H]	10 mM
9	D2O		99.8 %

3 HMQC-J

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CHITINASE A1	[U-15N]	2.0 mM
2	KH2PO4/K2HPO4		10 mM
3	dithiothreitol	[U-2H]	10 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
6	CHITINASE A1	[U-15N]	1.2 mM
7	KH2PO4/K2HPO4		100 mM
8	dithiothreitol	[U-2H]	10 mM
9	D2O		99.8 %

4 3D (H)NNH-TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CHITINASE A1	[U-15N]	2.0 mM
2	KH2PO4/K2HPO4		10 mM
3	dithiothreitol	[U-2H]	10 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
6	CHITINASE A1	[U-15N]	1.2 mM
7	KH2PO4/K2HPO4		100 mM
8	dithiothreitol	[U-2H]	10 mM
9	D2O		99.8 %

5 2D H(NN)H-TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CHITINASE A1	[U-15N]	2.0 mM
2	KH2PO4/K2HPO4		10 mM
3	dithiothreitol	[U-2H]	10 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
6	CHITINASE A1	[U-15N]	1.2 mM
7	KH2PO4/K2HPO4		100 mM
8	dithiothreitol	[U-2H]	10 mM
9	D2O		99.8 %

nullKeywords twisted beta-sandwich, CBD, cellulose, ChBD, chitin binding domain, ChiA1, chitinase A1, NMR

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.067 seconds)

BMRB: 4588

Sample

Related entities 1. ChBD-ChiA1, : 14 entities Detail

Experiments performed 5 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2