BMRB: 4589

NMR Solution Structure of the Last Unknown Module of the Cellulosomal Scaffoldin Protein CIPC of Clostridum cellulolyticum

Authors

Mosbah, A., Belaich, A., Bornet, O., Belaich, J.P., Henrissat, B., Darbon, H.

Assembly

scaffoldin protein

Entity

1. scaffoldin protein (polymer, Thiol state: not present), 94 monomers, 10035.17 Da Detail

MQDPTINPTS ISAKAGSFAD TKITLTPNGN TFNGISELQS SQYTKGTNEV TLLASYLNTL PENTTKTLTF DFGVGTKNPK LTITVLPKDI PGLE

Formula weight

10035.17 Da

Source organism

Ruminiclostridium cellulolyticum

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing and torsion angle dynamics

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 80.0 %, Completeness (bb): 97.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	80.0 % (515 of 644)	78.8 % (430 of 546)	86.7 % (85 of 98)
Backbone	97.5 % (268 of 275)	97.3 % (183 of 188)	97.7 % (85 of 87)
Sidechain	66.9 % (247 of 369)	69.0 % (247 of 358)	0.0 % (0 of 11)
Aromatic	0.0 % (0 of 28)	0.0 % (0 of 28)
Methyl	79.7 % (47 of 59)	79.7 % (47 of 59)

1. scaffoldin protein

MQDPTINPTS ISAKAGSFAD TKITLTPNGN TFNGISELQS SQYTKGTNEV TLLASYLNTL PENTTKTLTF DFGVGTKNPK LTITVLPKDI PGLE

Show sample condition

Sample

Pressure 1 atm, Temperature 300 K, pH 5.0

#	Name	Isotope labeling	Type	Concentration
1	scaffoldin protein	[U-15N]		25 mM
2	sodium acetate buffer			20 mM
3	H2O			90 %
4	D2O			10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 1EHX, Strand ID: A Detail

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Coupling constant

77 J values in 1 lists
Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 300 K, pH 5.0 Detail

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Pressure 1 atm, Temperature 300 K, pH 5.0

List #1 coupling_constants, Field strength (¹H) 500 MHz

Release date

2001-03-07

Citation

NMR Solution Structure of the Last Unknown Module of the Cellulosomal Scaffoldin Protein CIPC of Clostridum cellulolyticum
Mosbah, A., Belaich, A., Bornet, O., Belaich, J.P., Henrissat, B., Darbon, H.
J. Mol. Biol. (2000), 304, 201-217, PubMed , DOI:

Related entities 1. scaffoldin protein, : 1 : 3 entities Detail

Experiments performed 6 experiments Detail