BMRB: 4590

Solution structure of the human chemokine Eotaxin-2

Authors

Mayer, K.L., Stone, M.J.

Assembly

EOTAXIN-2

Entity

1. EOTAXIN-2 (polymer, Thiol state: all disulfide bound), 73 monomers, 8309.668 Da Detail

VVIPSPCCMF FVSKRIPENR VVSYQLSSRS TCLKAGVIFT TKKGQQSCGD PKQEWVQRYM KNLDAKQKKA SPR

Formula weight

8309.668 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	0:CYS7:SG	1:CYS32:SG
2	disulfide	sing	0:CYS8:SG	1:CYS48:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Hybrid distance geometry/simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 82.5 %, Completeness (bb): 85.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.5 % (732 of 887)	83.7 % (395 of 472)	76.9 % (260 of 338)	100.0 % (77 of 77)
Backbone	85.0 % (364 of 428)	99.3 % (143 of 144)	70.8 % (153 of 216)	100.0 % (68 of 68)
Sidechain	82.6 % (437 of 529)	76.8 % (252 of 328)	91.7 % (176 of 192)	100.0 % (9 of 9)
Aromatic	62.1 % (36 of 58)	62.1 % (18 of 29)	60.7 % (17 of 28)	100.0 % (1 of 1)
Methyl	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)

1. EOTAXIN-2

VVIPSPCCMF FVSKRIPENR VVSYQLSSRS TCLKAGVIFT TKKGQQSCGD PKQEWVQRYM KNLDAKQKKA SPR

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 4.1

#	Name	Isotope labeling	Type	Concentration
1	EOTAXIN-2	[U-15N; U-13C]		1 mM
2	NaAc			20 mM
3	H2O			90 %
4	D2O			10 %

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LACS Plot; CA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 1 models in PDB: 1EIG, Strand ID: A Detail

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Coupling constant

51 J values in 1 lists
Pressure 1 atm, Temperature 298 K, pH 4.1 Detail

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Pressure 1 atm, Temperature 298 K, pH 4.1

List #1 coupling_constants

Release date

2001-03-07

Citation

NMR solution structure and receptor peptide binding of the CC chemokine eotaxin-2
Mayer, K.L., Stone, M.J.
Biochemistry (2000), 39, 8382-8395, PubMed 10913244 , DOI: , Abstract

Related entities 1. EOTAXIN-2, : 1 : 1 : 2 : 246 entities Detail

Interaction partners 1. EOTAXIN-2, : 5 interactors Detail