BMRB: 4593

Rous sarcoma virus capsid protein: C-terminal domain

Authors

Kingston, R.L., Fitzon-Ostendorp, T., Eisenmesser, E.Z., Schatz, G.W., Vogt, V.M., Post, C.B., Rossmann, M.G.

Assembly

Rous sarcoma virus capsid protein: C-terminal domain

Entity

1. Rous sarcoma virus capsid protein: C-terminal domain (polymer, Thiol state: all free), 96 monomers, 10425.96 Da Detail

MDIMQGPSES FVDFANRLIK AVEGSDLPPS ARAPVIIDCF RQKSQPDIQQ LIRTAPSTLT TPGEIIKYVL DRQKTAPLTD QGIAAAMSSA IQPLIM

Formula weight

10425.96 Da

Source organism

Rous sarcoma virus - Prague C

Exptl. method

NMR

Refine. method

torsion angle dynamics/simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 80.2 %, Completeness: 72.6 %, Completeness (bb): 68.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	72.6 % (799 of 1101)	78.8 % (453 of 575)	62.8 % (270 of 430)	79.2 % (76 of 96)
Backbone	68.3 % (381 of 558)	77.0 % (144 of 187)	59.2 % (168 of 284)	79.3 % (69 of 87)
Sidechain	77.5 % (492 of 635)	79.6 % (309 of 388)	73.9 % (176 of 238)	77.8 % (7 of 9)
Aromatic	60.5 % (23 of 38)	100.0 % (19 of 19)	21.1 % (4 of 19)
Methyl	73.3 % (88 of 120)	73.3 % (44 of 60)	73.3 % (44 of 60)

1. RSV CA

MDIMQGPSES FVDFANRLIK AVEGSDLPPS ARAPVIIDCF RQKSQPDIQQ LIRTAPSTLT TPGEIIKYVL DRQKTAPLTD QGIAAAMSSA IQPLIM

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 K, pH 4.9

#	Name	Isotope labeling	Type	Concentration
1	RSV_CA	[U-15N]		1.5 mM
2	Sodium phosphate			50 mM
3	NaCl			50 mM
4	EDTA			1 mM
5	DTT			1 mM
6	H2O			90 %
7	D2O			10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 4.9

#	Name	Isotope labeling	Type	Concentration
8	RSV_CA	[U-15N; U-13C]		1.5 mM
9	Sodium phosphate			50 mM
10	NaCl			50 mM
11	EDTA			1 mM
12	DTT			1 mM
13	H2O			90 %
14	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.13 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.752 ppm	internal	direct	0.0
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.13 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.752 ppm	internal	direct	0.0
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.752 ppm	internal	direct	0.0
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 1 models in PDB: 1EOQ, Strand ID: A Detail

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Coupling constant

122 J values in 1 lists
Pressure 1 atm, Temperature 298 K, pH 4.9 Detail

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Pressure 1 atm, Temperature 298 K, pH 4.9

List #1 coupling_constants

Release date

2001-03-01

Citation

Structure and self-association of the Rous sarcoma virus capsid protein
Kingston, R.L., Fitzon-Ostendorp, T., Eisenmesser, E.Z., Schatz, G.W., Vogt, V.M., Post, C.B., Rossmann, M.G.
Structure (2000), 8, 617-628, PubMed 10873863 , DOI: , Abstract

Related entities 1. Rous sarcoma virus capsid protein: C-terminal domain, : 1 : 5 : 8 entities Detail

Experiments performed 4 experiments Detail