BMRB: 4601

Solution structure of the channel-former Zervamicin IIB (peptaibol antibiotic)

Authors

Balashova, T.A., Shenkarev, Z.O., Tagaev, A.A., Ovchinnikova, T.V., Raap, J., Arseniev, A.S.

Assembly

Zervamicin IIB

Entity

1. Zervamicin IIB (polymer, Thiol state: not present), 17 monomers, 1823.182 Da Detail

XWIQXITXLX XQXXXPX

Formula weight

1823.182 Da

Source organism

Emericellopsis salmosynnemata

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 47.1 %, Completeness: 98.2 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.2 % (109 of 111)	96.6 % (56 of 58)	100.0 % (43 of 43)	100.0 % (10 of 10)
Backbone	100.0 % (46 of 46)	100.0 % (15 of 15)	100.0 % (24 of 24)	100.0 % (7 of 7)
Sidechain	97.3 % (71 of 73)	95.3 % (41 of 43)	100.0 % (27 of 27)	100.0 % (3 of 3)
Aromatic	100.0 % (12 of 12)	100.0 % (6 of 6)	100.0 % (5 of 5)	100.0 % (1 of 1)
Methyl	100.0 % (14 of 14)	100.0 % (7 of 7)	100.0 % (7 of 7)

1. Zervamicin IIB

XWIQXITXLX XQXXXPX

Show sample condition

Sample

Pressure 1 atm, Temperature 303 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
1	Zervamicin IIB			10 mM

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Calculated from 20 models in PDB: 1DLZ, Strand ID: A Detail

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Coupling constant

59 J values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 303 K, pH 5.8 Detail

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Pressure 1 atm, Temperature 303 K, pH 5.8

List #1 J_values_set_1, Field strength (¹H) 600 MHz

Release date

2015-08-10

Citation

NMR structure of the channel-former zervamicin IIB in isotropic solvents
Balashova, T.A., Shenkarev, Z.O., Tagaev, A.A., Ovchinnikova, T.V., Raap, J., Arseniev, A.S.
FEBS Lett. (2000), 466, 333-336, PubMed 10682854 , DOI 10.1016/s0014-5793(99)01707-x , Abstract

Experiments performed 3 experiments Detail