BMRB: 4609

NMR observation of T-tetrads in a parallel stranded DNA quadruplex formed by Saccharomyces cerevisiae telomere sequence

Authors

Hosur, R.V., Patel, P.K.

Assembly

T-tetrad telomere repeats

Entity

1. T-tetrad telomere repeats (polymer, Thiol state: not present), 7 monomers, 2232.405 × 4 Da Detail

TGGTGGC

Total weight

8929.62 Da

Max. entity weight

2232.405 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 77.6 %, Completeness (bb): 75.5 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	77.6 % (52 of 67)	77.6 % (52 of 67)
Suger, PO4	75.5 % (37 of 49)	75.5 % (37 of 49)
Nucleobase	38.9 % (7 of 18)	38.9 % (7 of 18)
Aromatic	42.9 % (6 of 14)	42.9 % (6 of 14)
Methyl	50.0 % (1 of 2)	50.0 % (1 of 2)

1. yeast telomere repeats

TGGTGGC

Show sample condition

Sample

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	yeast telomere repeats			1.0 ~ 2.0 mM
2	potassium phosphate			10 mM
3	EDTA			0.2 mM
4	KCl			100 mM
5	H2O			90 %
6	D2O			10 %

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Release date

2001-01-03

Citation

NMR observation of T-tetrads in a parallel stranded DNA quadruplex formed by Saccharomyces cerevisiae telomere sequence
Hosur, R.V., Patel, P.K.
Nucleic Acids Res. (1999), 27, 2457-2464, PubMed , DOI:

Experiments performed 3 experiments Detail