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NMR observation of a novel C-tetrad in a DNA quadruplex
Authors
Patel, P.K., Bhavesh, N.S., Hosur, R.V.
Assembly
5'-D(*TP*GP*GP*GP*CP*GP*GP*T)-3'
Entity
1. 5'-D(*TP*GP*GP*GP*CP*GP*GP*T)-3' (polymer, Thiol state: not present), 8 monomers, 2561.611 × 4 Da Detail

TGGGCGGT


Total weight
10246.444 Da
Max. entity weight
2561.611 Da
Source organism
Macaca mulatta polyomavirus 1
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 76.3 %, Completeness (bb): 83.9 % Detail

Polymer type: polydeoxyribonucleotide

Total1H
All76.3 % (58 of 76)76.3 % (58 of 76)
Suger, PO483.9 % (47 of 56)83.9 % (47 of 56)
Nucleobase55.0 % (11 of 20)55.0 % (11 of 20)
Aromatic56.3 % (9 of 16)56.3 % (9 of 16)
Methyl100.0 % (2 of 2)100.0 % (2 of 2)

1. TGGGCGGT

TGGGCGGT

Sample

Pressure 1 atm, pH 7.0


#NameIsotope labelingTypeConcentration
1TGGGCGGT0.0 ~ 0.0 mM
2sodium phosphate10 mM
3EDTA0.2 mM
4NaCl200 mM
5H2O90 %
6D2O10 %

Release date
2000-12-18
Citation
NMR observation of a novel C-tetrad in the structure of the SV40 repeat sequence GGGCGG
Patel, P.K., Bhavesh, N.S., Hosur, R.V.
Biochem. Biophys. Res. Commun. (2000), 270, 967-971, PubMed 10772934 , DOI 10.1006/bbrc.2000.2479 ,
Experiments performed 3 experiments Detail
nullKeywords C-tetrad, GGGCGG repeat, G-quadruplex, NMR structure, SV40