BMRB: 4614

The G7(syn)-G4(anti) structure of r(GCAGGCGUGC)2

Authors

Burkard, M.E., Turner, D.H.

Assembly

5'-r(*GP*CP*AP*GP*GP*CP*GP*UP*GP*C)-3'

Entity

1. 5'-r(*GP*CP*AP*GP*GP*CP*GP*UP*GP*C)-3' (polymer, Thiol state: not present), 10 monomers, 3294.960 × 2 Da Detail

GCAGGCGUGC

Total weight

6589.92 Da

Max. entity weight

3294.96 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 62.1 %, Completeness (bb): 65.0 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	62.1 % (54 of 87)	62.1 % (54 of 87)
Suger, PO4	65.0 % (39 of 60)	65.0 % (39 of 60)
Nucleobase	55.6 % (15 of 27)	55.6 % (15 of 27)
Aromatic	71.4 % (15 of 21)	71.4 % (15 of 21)

1. 5'-r(*GP*CP*AP*GP*GP*CP*GP*UP*GP*C)-3'

GCAGGCGUGC

Show sample condition

Sample #1

Temperature 310.15 (±0.3) K, pH 7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	5'-r(*GP*CP*AP*GP*GP*CP*GP*UP*GP*C)-3'			3.0 mM
2	NaCl			80 mM
3	phosphate			10 mM
4	D2O			100 %

Sample #2

Temperature 310.15 (±0.3) K, pH 7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	5'-r(*GP*CP*AP*GP*GP*CP*GP*UP*GP*C)-3'			1.0 mM
6	NaCl			80 mM
7	Na_phosphate			10 mM
8	H2O			90 %
9	D2O			10 %

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Release date

2000-11-29

Citation

NMR structures of r(GCAGGCGUGC)2 and determinants of stability for single guanosine-guanosine base pairs
Burkard, M.E., Turner, D.H.
Biochemistry (2000), 39, 11748-11762, PubMed 10995243 , DOI 10.1021/bi000720i , Abstract

Experiments performed 2 experiments Detail