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BMRB: 4619

1E0E

N-terminal zinc-binding HHCC domain of HIV-2 integrase

Authors

Eijkelenboom, A.P.A.M., van den Ent, F.M.I., Plasterk, R.H.A., Kaptein, R., Boelens, R.

Assembly

Human immunodeficiency virus type 2 integrase

Entity

1. Human immunodeficiency virus type 2 integrase (polymer, Thiol state: all other bound), 55 monomers, 6255.956 × 2 Da Detail

FLEKIEPAQE EHEKYHSNVK ELSHKFGIPN LVARQIVNSC AQCQQKGEAI HGQVN

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

12642.73 Da

Max. entity weight

6255.956 Da

Entity Connection

metal coordination 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	2:ZN1:ZN	1:HIS12:NE2
2	metal coordination	sing	2:ZN1:ZN	1:HIS16:ND1
3	metal coordination	sing	2:ZN1:ZN	1:CYS40:SG
4	metal coordination	sing	2:ZN1:ZN	1:CYS43:SG
5	metal coordination	sing	2:ZN1:ZN	1:HIS12:NE2
6	metal coordination	sing	2:ZN1:ZN	1:HIS16:ND1
7	metal coordination	sing	2:ZN1:ZN	1:CYS40:SG
8	metal coordination	sing	2:ZN1:ZN	1:CYS43:SG

Source organism

Human immunodeficiency virus type 2 (ISOLATE ROD)

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.9 %, Completeness (bb): 85.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.9 % (597 of 657)	98.6 % (342 of 347)	77.7 % (192 of 247)	100.0 % (63 of 63)
Backbone	85.0 % (277 of 326)	98.2 % (109 of 111)	71.0 % (115 of 162)	100.0 % (53 of 53)
Sidechain	97.1 % (372 of 383)	98.7 % (233 of 236)	94.2 % (129 of 137)	100.0 % (10 of 10)
Aromatic	81.8 % (36 of 44)	100.0 % (22 of 22)	63.6 % (14 of 22)
Methyl	100.0 % (52 of 52)	100.0 % (26 of 26)	100.0 % (26 of 26)

1. Human immunodeficiency virus type 2 integrase

FLEKIEPAQE EHEKYHSNVK ELSHKFGIPN LVARQIVNSC AQCQQKGEAI HGQVN

Show sample condition

Sample

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.75 ppm	internal	direct	0.0
¹⁵C	DDS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³N	DDS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 1.91 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.75 ppm	internal	direct	0.0
¹⁵C	DDS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³N	DDS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 1.91 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.75 ppm	internal	direct	0.0
¹⁵C	DDS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³N	DDS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 32 models in PDB: 1E0E, Strand ID: A, B Detail

Release date

2000-12-04

Citation

Refined solution structure of the dimeric N-terminal HHCC domain of HIV-2 integrase
Eijkelenboom, A.P.A.M., van den Ent, F.M.I., Vos, A., Doreleijers, J.F., Hard, K., Tullius, T.D., Plasterk, R.H.A., Kaptein, R., Boelens, R.
J. Biomol. NMR (2000), 18, 119-128, PubMed 11101216 , Abstract

Related entities 1. Human immunodeficiency virus type 2 integrase, : 1 : 1 : 1 : 98 entities Detail

Interaction partners 1. Human immunodeficiency virus type 2 integrase, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 18 experiments Detail

1 2D (1H,15N)-HSQC

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

2 2D (1H,15N)-HSMQC

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

3 2D (1H,1H) NOESY

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

4 2D (1H,1H) TOCSY

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

5 3D TOCSY (15N,1H) HSQC

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

6 3D TOCSY (13C,1H) HSQC

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

7 3D NOESY (15N,1H) HSQC

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

8 3D NOESY (13C,1H) HSQC

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

9 3D (13C) HMQC NOESY (13C,1H) HSQC

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

10 2D 13C-filtered NOESY

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

11 3D 13C-filtered NOESY (13C,1H) HSQC

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

12 3D HNCO

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

13 3D HNCA

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

14 3D HN(CO)CA

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

15 3D HNHA

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

16 3D HNHB

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

17 3D H(C)CH DIPSI

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

18 3D HCC(H) DIPSI

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian Unity Plus (750 MHz)

Sample #1

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
2	ZINC (II) ION		1.1 eq
3	Tris	[U-2H]	50 mM
4	NaCl		150 mM
5	2-mercaptoethanol		2 mM
6	D2O		99.99 %

Sample #2

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Human immunodeficiency virus type 2 integrase	[U-15N]	3.5 mM
8	ZINC (II) ION		1.1 eq
9	Tris	[U-2H]	50 mM
10	NaCl		150 mM
11	2-mercaptoethanol		2 mM
12	H2O		95 %
13	D2O		5 %

Sample #3

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
15	ZINC (II) ION		1.1 eq
16	Tris	[U-2H]	50 mM
17	NaCl		150 mM
18	2-mercaptoethanol		2 mM
19	D2O		5 %

Sample #4

Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Human immunodeficiency virus type 2 integrase	[U-15N; U-13C]	3.5 mM
21	ZINC (II) ION		1.1 eq
22	Tris	[U-2H]	50 mM
23	NaCl		150 mM
24	2-mercaptoethanol		2 mM
25	H2O		95 %
26	D2O		5 %

nullKeywords integrase, AIDS, polyprotein, dimer, zinc-binding protein, helix-turn-helix

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Related entities 1. Human immunodeficiency virus type 2 integrase, : 1 : 1 : 1 : 98 entities Detail

Interaction partners 1. Human immunodeficiency virus type 2 integrase, : 1 interactors Detail

Experiments performed 18 experiments Detail

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