BMRB: 4622

Solution structure, Hydrodynamics and thermodynamics of the UvrB C-terminal domain

Authors

Alexandrovich, A., Czish, M., Frenkiel, T.A., Kelly, G.P., Goosen, N., Moolenaar, G.F., Chowdhry, B.Z., Sanderson, M.R., Lane, A.N.

Assembly

UvrB C-terminal domain

Entity

1. UvrB C-terminal domain (polymer, Thiol state: not present), 63 monomers, 7438.354 × 2 Da Detail

MHHHHHHLEP DNVPMDMSPK ALQQKIHELE GLMMQHAQNL EFEEAAQIRD QLHQLRELFI AAS

Total weight

14876.708 Da

Max. entity weight

7438.354 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.5 %, Completeness: 72.9 %, Completeness (bb): 73.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	72.9 % (553 of 759)	75.9 % (303 of 399)	64.9 % (189 of 291)	88.4 % (61 of 69)
Backbone	73.7 % (274 of 372)	87.9 % (109 of 124)	60.1 % (113 of 188)	86.7 % (52 of 60)
Sidechain	74.2 % (333 of 449)	70.5 % (194 of 275)	78.8 % (130 of 165)	100.0 % (9 of 9)
Aromatic	21.4 % (12 of 56)	21.4 % (6 of 28)	21.4 % (6 of 28)
Methyl	91.7 % (55 of 60)	93.3 % (28 of 30)	90.0 % (27 of 30)

1. UvrB C-terminal domain

MHHHHHHLEP DNVPMDMSPK ALQQKIHELE GLMMQHAQNL EFEEAAQIRD QLHQLRELFI AAS

Show sample condition

Sample

Temperature 293 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	UvrB C-terminal domain	[U-13C; U-15N]		10 mg/mL

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Protein Blocks Logo

Calculated from 20 models in PDB: 1E52, Strand ID: A, B Detail

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Release date

2002-04-03

Citation

Solution structure, hydrodynamics and thermodynamics of the UvrB C-terminal domain
Alexandrovich, A., Czisch, M., Frenkiel, T.A., Kelly, G.P., Goosen, N., Moolenaar, G.F., Chowdhry, B.Z., Sanderson, M.R., Lane, A.N.
J. Biomol. Struct. Dyn. (2001), 19, 219-236, PubMed 11697728 , DOI 10.1080/07391102.2001.10506734 , Abstract

Related entities 1. UvrB C-terminal domain, : 1 : 1 : 22 entities Detail

Interaction partners 1. UvrB C-terminal domain, : 29 interactors Detail

Experiments performed 3 experiments Detail