BMRB: 4623

Internal xylan binding domain from C. fimi Xyn10A, R262G mutant

Authors

Simpson, P.J., Hefang, X., Bolam, D.N., Gilbert, H.J., Williamson, M.P.

Assembly

XYLANASE D

Entity

1. XYLANASE D (polymer, Thiol state: all disulfide bound), 87 monomers, 8684.245 Da Detail

TGCSVTATRA EEWSDGFNVT YSVSGSSAWT VNLALNGSQT IQASWNANVT GSGSTRTVTP NGSGNTFGVT VMKNGSSTTP AATCAGS

Formula weight

8684.245 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS3:SG	1:CYS84:SG

Source organism

Cellulomonas fimi

Exptl. method

NMR

Refine. method

HYBRID DISTANCE GEOMETRY/ SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 98.3 %, Completeness (bb): 98.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	98.3 % (519 of 528)	98.1 % (422 of 430)	99.0 % (97 of 98)
Backbone	98.1 % (263 of 268)	97.8 % (179 of 183)	98.8 % (84 of 85)
Sidechain	98.5 % (256 of 260)	98.4 % (243 of 247)	100.0 % (13 of 13)
Aromatic	100.0 % (35 of 35)	100.0 % (32 of 32)	100.0 % (3 of 3)
Methyl	95.7 % (44 of 46)	95.7 % (44 of 46)

1. XYLANASE D

TGCSVTATRA EEWSDGFNVT YSVSGSSAWT VNLALNGSQT IQASWNANVT GSGSTRTVTP NGSGNTFGVT VMKNGSSTTP AATCAGS

Show sample condition

Sample

Temperature 310 K, pH 5.0

#	Name	Isotope labeling	Type	Concentration
1	XYLANASE D	[U-15N]		1.5 mM

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Protein Blocks Logo

Calculated from 5 models in PDB: 1E5C, Strand ID: A Detail

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Release date

2000-09-06

Citation

The structural basis for the ligand specificity of family 2 carbohydrate-binding modules
Simpson, P.J., Xie, H., Bolam, D.N., Gilbert, H.J., Williamson, M.P.
J. Biol. Chem. (2000), 275, 41137-41142, PubMed 10973978 , DOI 10.1074/jbc.M006948200 , Abstract

Related entities 1. XYLANASE D, : 1 : 1 : 3 : 14 entities Detail

Experiments performed 9 experiments Detail