BMRB: 4628

Heavy Chain Variable domain from LLama

Authors

Renisio, J., Perez, J., Czisch, M., Guenneugues, M., Bornet, O., Frenken, L., Cambillau, C., Darbon, H.

Assembly

H14

Entity

1. H14 (polymer, Thiol state: all disulfide bound), 117 monomers, 12509.61 Da Detail

QVQLQESGGG LVQAGGSLRL SCAASGRTGS TYDMGWFRQA PGKERESVAA INWDSARTYY ASSVRGRFTI SRDNAKKTVY LQMNSLKPED TAVYTCGAGE GGTWDSWGQG TQVTVSS

Formula weight

12509.61 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS22:SG	1:CYS96:SG

Source organism

Lama glama

Exptl. method

NMR

Refine. method

distance geometry, molecular dynamics

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 88.5 %, Completeness (bb): 97.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	88.5 % (576 of 651)	88.5 % (576 of 651)
Backbone	97.2 % (241 of 248)	97.2 % (241 of 248)
Sidechain	83.1 % (335 of 403)	83.1 % (335 of 403)
Aromatic	61.1 % (33 of 54)	61.1 % (33 of 54)
Methyl	92.5 % (49 of 53)	92.5 % (49 of 53)

1. H14

QVQLQESGGG LVQAGGSLRL SCAASGRTGS TYDMGWFRQA PGKERESVAA INWDSARTYY ASSVRGRFTI SRDNAKKTVY LQMNSLKPED TAVYTCGAGE GGTWDSWGQG TQVTVSS

Show sample condition

Sample

Pressure 1 atm, Temperature 300 K, pH 4.6

#	Name	Isotope labeling	Type	Concentration
1	H14	[U-15N]		3 mM
2	Na acetate	[U-2H]		10 mM
3	H2O			90 %
4	D2O			10 %

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Calculated from 20 models in PDB: 1G9E, Strand ID: A Detail

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Coupling constant

46 J values in 1 lists
Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 300 K, pH 4.6 Detail

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Pressure 1 atm, Temperature 300 K, pH 4.6

List #1 coupling_constants_set_1, Field strength (¹H) 500 MHz

Release date

2001-03-09

Citation

Solution structure and backbone dynamics of an antigen-free heavy chain variable domain (VHH) from Llama
Renisio, J., Perez, J., Czisch, M., Guenneugues, M., Bornet, O., Frenken, L., Cambillau, C., Darbon, H.
Proteins: Struct., Funct., Genet. (2002), 47, 546-555

Related entities 1. H14, : 1 : 1 : 430 entities Detail

Experiments performed 1 experiments Detail