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Found 1 Document (4.357 seconds)

BMRB: 4635

1G1E

Solution structure of the interacting domains of the Mad-Sin3 complex: implications for recruitment of a chromatin-modifying complex

Authors: Brubaker, K., Cowley, S.M., Huang, K., Eisenman, R.N., Radhakrishnan, I.
Assembly: MAD1 PROTEIN/SIN3A
Entity: 1. MAD1 SID domain (polymer, Thiol state: not present), 16 monomers, 1966.285 Da Detail
RMNIQMLLEA ADYLER

2. mSin3A PAH2 Domain (polymer, Thiol state: not present), 89 monomers, 10332.35 Da Detail
SLQNNQPVEF NHAINYVNKI KNRFQGQPDI YKAFLEILHT YQKEQRNAKE AGGNYTPALT EQEVYAQVAR LFKNQEDLLS EFGQFLPDA
Total weight: 12298.635 Da
Max. entity weight: 10332.35 Da
Exptl. method: NMR
Refine. method: distance geometry, simulated annealing
Data set: coupling_constants
Protein Blocks Logo: Calculated from 15 models in PDB: 1G1E, Strand ID: A, B Detail
Coupling constant: 72 J values in 1 lists
Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 300 K, pH 6.0 Detail
Pressure 1 atm, Temperature 300 K, pH 6.0
List #1 JHNHA, Field strength (¹H) 500 MHz
Release date: 2001-05-06
Citation: Solution structure of the interacting domains of the Mad-Sin3 complex: implications for recruitment of a chromatin-modifying complex
Brubaker, K., Cowley, S.M., Huang, K., Loo, L., Yochum, G.S., Ayer, D.E., Eisenman, R.N., Radhakrishnan, I.
Cell (2000), 103, 655-665, PubMed 11106735 , DOI: , Abstract

Related entities 1. MAD1 SID domain, : 1 : 4 : 1 : 9 entities Detail

Related entities 2. mSin3A PAH2 Domain, : 5 : 126 entities Detail

Interaction partners 1. MAD1 SID domain, : 6 : 1 interactors Detail

More details for 7 interactors identified by 5 citations

Interaction partners 2. mSin3A PAH2 Domain, : 33 interactors Detail

More details for 33 interactors identified by 21 citations

Experiments performed 7 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	MAD1 SID domain		1.0 mM
2	mSin3A PAH2 Domain	[U-15N]	1.0 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	MAD1 SID domain		1.6 mM
6	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	MAD1 SID domain		1.6 mM
10	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
11	H2O		90 %
12	D2O		10 %
13	phosphate buffer		20 mM
14	NaN3		0.2 %

Sample #4

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
15	MAD1 SID domain		1.6 mM
16	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
17	D2O		100 %
18	phosphate buffer		20 mM
19	NaN3		0.2 %

2 HNHA

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	MAD1 SID domain		1.0 mM
2	mSin3A PAH2 Domain	[U-15N]	1.0 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	MAD1 SID domain		1.6 mM
6	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	MAD1 SID domain		1.6 mM
10	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
11	H2O		90 %
12	D2O		10 %
13	phosphate buffer		20 mM
14	NaN3		0.2 %

Sample #4

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
15	MAD1 SID domain		1.6 mM
16	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
17	D2O		100 %
18	phosphate buffer		20 mM
19	NaN3		0.2 %

3 2D 13C,15N-double-half-filtered NOESY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	MAD1 SID domain		1.0 mM
2	mSin3A PAH2 Domain	[U-15N]	1.0 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	MAD1 SID domain		1.6 mM
6	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	MAD1 SID domain		1.6 mM
10	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
11	H2O		90 %
12	D2O		10 %
13	phosphate buffer		20 mM
14	NaN3		0.2 %

Sample #4

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
15	MAD1 SID domain		1.6 mM
16	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
17	D2O		100 %
18	phosphate buffer		20 mM
19	NaN3		0.2 %

4 2D 13C-double-half-filtered NOESY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	MAD1 SID domain		1.0 mM
2	mSin3A PAH2 Domain	[U-15N]	1.0 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	MAD1 SID domain		1.6 mM
6	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	MAD1 SID domain		1.6 mM
10	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
11	H2O		90 %
12	D2O		10 %
13	phosphate buffer		20 mM
14	NaN3		0.2 %

Sample #4

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
15	MAD1 SID domain		1.6 mM
16	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
17	D2O		100 %
18	phosphate buffer		20 mM
19	NaN3		0.2 %

5 3D 13C-separated_NOESY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	MAD1 SID domain		1.0 mM
2	mSin3A PAH2 Domain	[U-15N]	1.0 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	MAD1 SID domain		1.6 mM
6	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	MAD1 SID domain		1.6 mM
10	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
11	H2O		90 %
12	D2O		10 %
13	phosphate buffer		20 mM
14	NaN3		0.2 %

Sample #4

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
15	MAD1 SID domain		1.6 mM
16	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
17	D2O		100 %
18	phosphate buffer		20 mM
19	NaN3		0.2 %

6 3D HACAHB

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	MAD1 SID domain		1.0 mM
2	mSin3A PAH2 Domain	[U-15N]	1.0 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	MAD1 SID domain		1.6 mM
6	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	MAD1 SID domain		1.6 mM
10	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
11	H2O		90 %
12	D2O		10 %
13	phosphate buffer		20 mM
14	NaN3		0.2 %

Sample #4

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
15	MAD1 SID domain		1.6 mM
16	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
17	D2O		100 %
18	phosphate buffer		20 mM
19	NaN3		0.2 %

7 3D HNHB

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	MAD1 SID domain		1.0 mM
2	mSin3A PAH2 Domain	[U-15N]	1.0 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
5	MAD1 SID domain		1.6 mM
6	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	MAD1 SID domain		1.6 mM
10	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
11	H2O		90 %
12	D2O		10 %
13	phosphate buffer		20 mM
14	NaN3		0.2 %

Sample #4

Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
15	MAD1 SID domain		1.6 mM
16	mSin3A PAH2 Domain	[U-15N; U-13C]	1.6 mM
17	D2O		100 %
18	phosphate buffer		20 mM
19	NaN3		0.2 %

nullKeywords Four-helix bundle, Protein-peptide Complex

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Found 1 Document (4.357 seconds)

BMRB: 4635

Related entities 1. MAD1 SID domain, : 1 : 4 : 1 : 9 entities Detail

Related entities 2. mSin3A PAH2 Domain, : 5 : 126 entities Detail

Interaction partners 1. MAD1 SID domain, : 6 : 1 interactors Detail

Interaction partners 2. mSin3A PAH2 Domain, : 33 interactors Detail

Experiments performed 7 experiments Detail

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