BMRBj - BMREntryMaster

Found 1 Document (2.611 seconds)

BMRB: 4636

1F2H

Solution structure of the N-terminal domain of the TNFR1 associated protein, TRADD

Authors

Tsao, D., McDonaugh, T., Malakian, K., Xu, G., Telliez, J., Hsu, H., Lin, L.

Assembly

Tumor necrosis factor receptor type 1 associated death domain protein

Entity

1. Tumor necrosis factor receptor type 1 associated death domain protein (polymer, Thiol state: all free), 169 monomers, 18547.95 Da Detail

MAAGQNGHEE WVGSAYLFVE SSLDKVVLSD AYAHPQQKVA VYRALQAALA ESGGSPDVLQ MLKIHRSDPQ LIVQLRFCGR QPCGRFLRAY REGALRAALQ RSLAAALAQH SVPLQLELRA GAERLDALLA DEERCLSCIL AQQPDRLRDE ELAELEDALR NLKCGSGAR

Formula weight

18547.95 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Distance geometry/simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 83.3 %, Completeness (bb): 81.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.3 % (1588 of 1906)	86.8 % (862 of 993)	75.9 % (557 of 734)	94.4 % (169 of 179)
Backbone	81.7 % (819 of 1002)	94.8 % (325 of 343)	68.5 % (340 of 496)	94.5 % (154 of 163)
Sidechain	86.7 % (921 of 1062)	82.6 % (537 of 650)	93.2 % (369 of 396)	93.8 % (15 of 16)
Aromatic	91.1 % (82 of 90)	91.1 % (41 of 45)	90.9 % (40 of 44)	100.0 % (1 of 1)
Methyl	96.2 % (200 of 208)	97.1 % (101 of 104)	95.2 % (99 of 104)

1. TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN

MAAGQNGHEE WVGSAYLFVE SSLDKVVLSD AYAHPQQKVA VYRALQAALA ESGGSPDVLQ MLKIHRSDPQ LIVQLRFCGR QPCGRFLRAY REGALRAALQ RSLAAALAQH SVPLQLELRA GAERLDALLA DEERCLSCIL AQQPDRLRDE ELAELEDALR NLKCGSGAR

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
1	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N]		1.0 mM
2	NaCl			200 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
3	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-13C]		1.1 mM
4	NaCl			200 mM

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
5	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N; U-13C]		0.8 mM
6	NaCl			200 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹⁵N	na	na	null	null
¹³C	na	na	null	null

Referencing offset: 0.34 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹⁵N	na	na	null	null
¹³C	na	na	null	null

Referencing offset: 0.34 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹⁵N	na	na	null	null
¹³C	na	na	null	null

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 1F2H, Strand ID: A Detail

Release date

2001-07-29

Citation

Solution structure of N-TRADD and characterization of the interaction of N-TRADD and C-TRAF2, a key step in the TNFR1 signaling pathway
Tsao, D., McDonaugh, T., Malakian, K., Xu, G., Telliez, J., Hsu, H., Lin, L.
Mol. Cell (2000), 5, 1051-1057, PubMed 10911999 , DOI: , Abstract

Related entities 1. Tumor necrosis factor receptor type 1 associated death domain protein, : 1 : 3 : 8 entities Detail

Interaction partners 1. Tumor necrosis factor receptor type 1 associated death domain protein, : 60 interactors Detail

More details for 60 interactors identified by 15 citations

Experiments performed 10 experiments Detail

1 3D 13C-separated NOESY

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
1	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N]		1.0 mM
2	NaCl			200 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
3	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-13C]		1.1 mM
4	NaCl			200 mM

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
5	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N; U-13C]		0.8 mM
6	NaCl			200 mM

2 3D 15N-separated NOESY

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
1	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N]		1.0 mM
2	NaCl			200 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
3	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-13C]		1.1 mM
4	NaCl			200 mM

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
5	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N; U-13C]		0.8 mM
6	NaCl			200 mM

3 HNHA

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
1	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N]		1.0 mM
2	NaCl			200 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
3	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-13C]		1.1 mM
4	NaCl			200 mM

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
5	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N; U-13C]		0.8 mM
6	NaCl			200 mM

4 HNCACB

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
1	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N]		1.0 mM
2	NaCl			200 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
3	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-13C]		1.1 mM
4	NaCl			200 mM

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
5	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N; U-13C]		0.8 mM
6	NaCl			200 mM

5 HN(CO)CACB

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
1	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N]		1.0 mM
2	NaCl			200 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
3	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-13C]		1.1 mM
4	NaCl			200 mM

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
5	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N; U-13C]		0.8 mM
6	NaCl			200 mM

6 C(CO)NH-TOCSY

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
1	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N]		1.0 mM
2	NaCl			200 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
3	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-13C]		1.1 mM
4	NaCl			200 mM

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
5	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N; U-13C]		0.8 mM
6	NaCl			200 mM

7 HCCH TOCSY

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
1	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N]		1.0 mM
2	NaCl			200 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
3	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-13C]		1.1 mM
4	NaCl			200 mM

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
5	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N; U-13C]		0.8 mM
6	NaCl			200 mM

8 HAHB(CO)NH

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
1	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N]		1.0 mM
2	NaCl			200 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
3	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-13C]		1.1 mM
4	NaCl			200 mM

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
5	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N; U-13C]		0.8 mM
6	NaCl			200 mM

9 15N-edited TOCSY-HSQC

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
1	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N]		1.0 mM
2	NaCl			200 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
3	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-13C]		1.1 mM
4	NaCl			200 mM

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
5	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N; U-13C]		0.8 mM
6	NaCl			200 mM

10 methyl-methyl NOESY

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
1	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N]		1.0 mM
2	NaCl			200 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
3	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-13C]		1.1 mM
4	NaCl			200 mM

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.6

#	Name	Isotope labeling	Type	Concentration
5	TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN	[U-15N; U-13C]		0.8 mM
6	NaCl			200 mM

nullKeywords TNFR-1 associated protein

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Found 1 Document (2.611 seconds)

BMRB: 4636

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Chemical shift reference

Chemical shift reference

Related entities 1. Tumor necrosis factor receptor type 1 associated death domain protein, : 1 : 3 : 8 entities Detail

Interaction partners 1. Tumor necrosis factor receptor type 1 associated death domain protein, : 60 interactors Detail

Experiments performed 10 experiments Detail

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