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SOLUTION STRUCTURE OF THE MATA1 HOMEODOMAIN
Authors
Anderson, J.S., Forman, M., Modleski, S., Dahlquist, F.W., Baxter, S.M.
Assembly
MATING-TYPE PROTEIN A-1
Entity
1. MATING-TYPE PROTEIN A-1 (polymer, Thiol state: all free), 61 monomers, 7146.347 Da Detail

KKEKSPKGKS SISPQARAFL EQVFRRKQSL NSKEKEEVAK KCGITPLQVR VWFINKRMRS K


Formula weight
7146.347 Da
Source organism
Saccharomyces cerevisiae
Exptl. method
NMR
Refine. method
distance geometry and simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 62.2 %, Completeness (bb): 82.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All62.2 % (488 of 784)72.1 % (307 of 426)42.0 % (123 of 293)89.2 % (58 of 65)
Backbone82.2 % (296 of 360)95.0 % (115 of 121)68.0 % (123 of 181)100.0 % (58 of 58)
Sidechain51.8 % (250 of 483)63.0 % (192 of 305)33.9 % (58 of 171) 0.0 % (0 of 7)
Aromatic40.5 % (17 of 42)81.0 % (17 of 21) 0.0 % (0 of 20) 0.0 % (0 of 1)
Methyl47.9 % (23 of 48)83.3 % (20 of 24)12.5 % (3 of 24)

1. MATING-TYPE PROTEIN A-1

KKEKSPKGKS SISPQARAFL EQVFRRKQSL NSKEKEEVAK KCGITPLQVR VWFINKRMRS K

Sample

Pressure 1 atm, Temperature 298 K, pH 4.5


#NameIsotope labelingTypeConcentration
1MATING-TYPE PROTEIN A-1[U-15N; U-13C]1.5 mM
2acetate[U-2H]25 mM
3KCl100 mM
4NaN30.01 %
5H2O90 %
6D2O10 %

LACS Plot; CA
Referencing offset: 3.32 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 3.32 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.1 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 1F43, Strand ID: A Detail


Release date
2001-07-08
Citation
Cooperative ordering in homeodomain-DNA recognition: solution structure and dynamics of the MATa1 homeodomain
Anderson, J.S., Forman, M., Modleski, S., Dahlquist, F.W., Baxter, S.M.
Biochemistry (2000), 39, 10045-10054, PubMed 10955992 , DOI: ,
Entries sharing articles Swiss-Prot: 1 entries Detail
  Swiss-Prot: P0CY10 released on 2011-06-28
    Title MATA1_YEASX Entity Mating-type protein A1
Related entities 1. MATING-TYPE PROTEIN A-1, : 1 : 3 : 1 : 1 : 186 entities Detail
Interaction partners 1. MATING-TYPE PROTEIN A-1, : 1 interactors Detail
Experiments performed 5 experiments Detail
nullKeywords Homeodomain, helix-turn-helix