BMRB: 4641

Human Prion Protein Mutant E200K Fragment 90-231

Authors

Zhang, Y., Swietnicki, W., Zagorski, M.G., Surewicz, W.K., Soennichsen, F.D.

Assembly

HUMAN PRION PROTEIN (MUTANT E200K)

Entity

1. HUMAN PRION PROTEIN (MUTANT E200K) (polymer, Thiol state: all disulfide bound), 146 monomers, 16503.20 Da Detail

GSDPGQGGGT HSQWNKPSKP KTNMKHMAGA AAAGAVVGGL GGYMLGSAMS RPIIHFGSDY EDRYYRENMH RYPNQVYYRP MDEYSNQNNF VHDCVNITIK QHTVTTTTKG ENFTKTDVKM MERVVEQMCI TQYERESQAY YQRGSS

Formula weight

16503.2 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS94:SG	1:CYS129:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.2 %, Completeness (bb): 96.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.2 % (1552 of 1666)	93.1 % (811 of 871)	92.6 % (589 of 636)	95.6 % (152 of 159)
Backbone	96.8 % (836 of 864)	97.7 % (294 of 301)	96.7 % (409 of 423)	95.0 % (133 of 140)
Sidechain	90.6 % (845 of 933)	90.7 % (517 of 570)	89.8 % (309 of 344)	100.0 % (19 of 19)
Aromatic	64.9 % (100 of 154)	66.2 % (51 of 77)	63.2 % (48 of 76)	100.0 % (1 of 1)
Methyl	99.0 % (101 of 102)	100.0 % (51 of 51)	98.0 % (50 of 51)

1. PRION PROTEIN

GSDPGQGGGT HSQWNKPSKP KTNMKHMAGA AAAGAVVGGL GGYMLGSAMS RPIIHFGSDY EDRYYRENMH RYPNQVYYRP MDEYSNQNNF VHDCVNITIK QHTVTTTTKG ENFTKTDVKM MERVVEQMCI TQYERESQAY YQRGSS

Show sample condition

Sample

Pressure 1 atm, Temperature 299 K, pH 4.6

#	Name	Isotope labeling	Type	Concentration
1	PRION PROTEIN	[U-15N; U-13C]		0.8 mM
2	acetate buffer			10 mM
3	H2O			90 %
4	D2O			10 %

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LACS Plot; CA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CO

Referencing offset: -0.08 ppm, Outliers: 5 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 30 models in PDB: 1FO7, Strand ID: A Detail

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Coupling constant

44 J values in 1 lists
Field strength (¹H) 800 MHz, Pressure 1 atm, Temperature 299 K, pH 4.6 Detail

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Pressure 1 atm, Temperature 299 K, pH 4.6

List #1 J_values, Field strength (¹H) 800 MHz

Release date

2001-05-16

Citation

Solution structure of Human Prion Protein Mutant E200K Fragment 90-231
Zhang, Y., Swietnicki, W., Zagorski, M.G., Surewicz, W.K., Soennichsen, F.D.
J. Biol. Chem. (2000), 275, 33650-33654, DOI 10.2210/pdb1fo7/pdb

Related entities 1. HUMAN PRION PROTEIN (MUTANT E200K), : 1 : 2 : 1 : 97 entities Detail

Interaction partners 1. HUMAN PRION PROTEIN (MUTANT E200K), : 73 interactors Detail

Experiments performed 4 experiments Detail