BMRB: 4643

The solution structure of sheep myeloid antimicrobial peptide (smap29) and its relationship to biological function

Authors

Waring, A.J., Sawai, M.V., McCray, P.B., Robertson, A.D., Lehrer, R.I.

Assembly

MYELOID ANTIMICROBIAL PEPTIDE

Entity

1. MYELOID ANTIMICROBIAL PEPTIDE (polymer, Thiol state: not present), 29 monomers, 3255.956 Da Detail

RGLRRLGRKI AHGVKKYGPT VLRIIRIAG

Formula weight

3255.956 Da

Source organism

Ovis aries

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 96.6 %, Completeness: 87.8 %, Completeness (bb): 96.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	87.8 % (166 of 189)	87.8 % (166 of 189)
Backbone	96.8 % (60 of 62)	96.8 % (60 of 62)
Sidechain	83.5 % (106 of 127)	83.5 % (106 of 127)
Aromatic	100.0 % (6 of 6)	100.0 % (6 of 6)
Methyl	100.0 % (21 of 21)	100.0 % (21 of 21)

1. MYELOID ANTIMICROBIAL PEPTIDE

RGLRRLGRKI AHGVKKYGPT VLRIIRIAG

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5.94

#	Name	Isotope labeling	Type	Concentration
1	MYELOID ANTIMICROBIAL PEPTIDE			1 mM
2	phosphate buffer			50 mM
3	H2O			60 %
4	trifluoroethanol	[U-2H]		40 %

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Calculated from 40 models in PDB: 1FRY, Strand ID: A Detail

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Coupling constant

8 J values in 1 lists
Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 298 K, pH 5.94 Detail

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Pressure 1 atm, Temperature 298 K, pH 5.94

List #1 JHNHA, Field strength (¹H) 500 MHz

Release date

2002-09-22

Citation

SMAP-29 has two LPS-binding sites and a central hinge
Tack, B.F., Sawai, M.V., Kearny, W.R., Robertson, A.D., Sherman, M.A., Wang, W., Hong, T., Boo, L.M., Wu, H., Waring, A.J., Lehrer, R.I.
Eur. J. Biochem. (2002), 269, 1181-1189, PubMed 11856344 , DOI 10.1046/j.0014-2956.2002.02751.x , Abstract

Related entities 1. MYELOID ANTIMICROBIAL PEPTIDE, : 1 : 2 : 1 entities Detail

Experiments performed 3 experiments Detail