BMRB: 4644

Solution structures of two CCHC zinc fingers from the FOG family protein U-shaped that mediate protein-protein interactions

Authors

Liew, C.K., Kowalski, K., Fox, A.H., Newton, A., Sharpe, B.K., Crossley, M., Mackay, J.P.

Assembly

FIRST ZINC FINGER FROM THE DROSOPHILA U-SHAPED TRANSCRIPTION FACTOR

Entity

1. FIRST ZINC FINGER OF U-SHAPED (polymer, Thiol state: all other bound), 36 monomers, 3956.594 Da Detail

GSLLKPARFM CLPCGIAFSS PSTLEAHQAY YCSHRI

2. ZN (non-polymer), 65.409 Da

Total weight

4022.003 Da

Max. entity weight

3956.594 Da

Entity Connection

metal coordination 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:CYS11:SG	2:ZN1:ZN
2	metal coordination	sing	1:CYS14:SG	2:ZN1:ZN
3	metal coordination	sing	1:CYS32:SG	2:ZN1:ZN
4	metal coordination	sing	1:HIS27:NE2	2:ZN1:ZN

Source organism

Drosophila melanogaster

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 87.4 %, Completeness (bb): 94.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	87.4 % (215 of 246)	86.3 % (183 of 212)	94.1 % (32 of 34)
Backbone	94.2 % (98 of 104)	94.4 % (67 of 71)	93.9 % (31 of 33)
Sidechain	82.4 % (117 of 142)	82.3 % (116 of 141)	100.0 % (1 of 1)
Aromatic	95.5 % (21 of 22)	95.5 % (21 of 22)
Methyl	100.0 % (17 of 17)	100.0 % (17 of 17)

1. FIRST ZINC FINGER OF U-SHAPED

GSLLKPARFM CLPCGIAFSS PSTLEAHQAY YCSHRI

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	FIRST ZINC FINGER OF U-SHAPED			0.7 mM
2	H2O			95 %
3	D2O			5 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 1FV5, Strand ID: A Detail

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Coupling constant

28 J values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 5.5 Detail

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Pressure 1 atm, Temperature 298 K, pH 5.5

List #1 JHNHA, Field strength (¹H) 600 MHz

Release date

2001-05-08

Citation

Solution structures of two CCHC zinc fingers from the FOG family protein U-shaped that mediate protein-protein interactions
Liew, C.K., Kowalski, K., Fox, A.H., Newton, A., Sharpe, B.K., Crossley, M., Mackay, J.P.
Structure (2000), 8, 1157-1166, PubMed 11080638 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. FIRST ZINC FINGER OF U-SHAPED, : 1 : 2 : 2 : 61 entities Detail

Experiments performed 3 experiments Detail