BMRB: 4648

Solution Structure and Dynamics of an Open B-sheet, Glycolytic Enzyme-monomeric 23.7 kDa Phosphoglycerate Mutase from Schizosaccharomyces pombe

Authors

Uhrinova, S., Uhrin, D., Nairn, J., Price, N.C., Fothergill-Gilmore, L.A.

Assembly

Phosphoglycerate Mutase (E.C.5.4.2.1)

Entity

1. Phosphoglycerate Mutase (E.C.5.4.2.1) (polymer, Thiol state: all free), 211 monomers, 23764.82 Da Detail

MTTEAAPNLL VLTRHGESEW NKLNLFTGWK DPALSETGIK EAKLGGERLK SRGYKFDIAF TSALQRAQKT CQIILEEVGE PNLETIKSEK LNERYYGDLQ GLNKDDARKK WGAEQVQIWR RSYDIAPPNG ESLKDTAERV LPYYKSTIVP HILKGEKVLI AAHGNSLRAL IMDLEGLTGD QIVKRELATG VPIVYHLDKD GKYVSKELID N

Formula weight

23764.82 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 75.5 %, Completeness (bb): 82.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	75.5 % (1885 of 2498)	77.7 % (1016 of 1307)	70.4 % (681 of 968)	84.3 % (188 of 223)
Backbone	82.1 % (1026 of 1250)	95.1 % (410 of 431)	69.6 % (429 of 616)	92.1 % (187 of 203)
Sidechain	72.1 % (1039 of 1442)	69.2 % (606 of 876)	79.1 % (432 of 546)	5.0 % (1 of 20)
Aromatic	38.6 % (61 of 158)	57.0 % (45 of 79)	21.3 % (16 of 75)	0.0 % (0 of 4)
Methyl	99.2 % (248 of 250)	98.4 % (123 of 125)	100.0 % (125 of 125)

1. Phosphoglycerate Mutase

MTTEAAPNLL VLTRHGESEW NKLNLFTGWK DPALSETGIK EAKLGGERLK SRGYKFDIAF TSALQRAQKT CQIILEEVGE PNLETIKSEK LNERYYGDLQ GLNKDDARKK WGAEQVQIWR RSYDIAPPNG ESLKDTAERV LPYYKSTIVP HILKGEKVLI AAHGNSLRAL IMDLEGLTGD QIVKRELATG VPIVYHLDKD GKYVSKELID N

Show sample condition

Sample

Pressure 1 atm, Temperature 310 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	Phosphoglycerate Mutase			1 mM
2	sodium acetate			200 mM
3	ammonium sulphate			200 mM
4	H2O			90 %
5	D2O			10 %

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LACS Plot; CA

Referencing offset: 0.29 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: 0.29 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 21 models in PDB: 1FZT, Strand ID: A Detail

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Coupling constant

203 J values in 1 lists
Field strength (¹H) 800 MHz, Pressure 1 atm, Temperature 310 K, pH 6.4 Detail

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Pressure 1 atm, Temperature 310 K, pH 6.4

List #1 coupling_constants, Field strength (¹H) 800 MHz

Release date

2001-05-08

Citation

Solution Structure and Dynamics of an Open B-sheet, Glycolytic Enzyme-monomeric 23.7 kDa Phosphoglycerate Mutase from Schizosaccharomyces pombe
Uhrinova, S., Uhrin, D., Nairn, J., Price, N.C., Fothergill-Gilmore, L.A.
J. Mol. Biol. (2001), 306, 275-290, PubMed , DOI:

Related entities 1. Phosphoglycerate Mutase (E.C.5.4.2.1), : 1 : 2 : 235 entities Detail

Interaction partners 1. Phosphoglycerate Mutase (E.C.5.4.2.1), : 1 interactors Detail

Experiments performed 3 experiments Detail