BMRB: 4650

Sheep prion protein synthetic peptide spanning helix 1 and b strand 2 (residues 142 to 166) shows b hairpin structure in solution

Authors

Kozin, S.A., Bertho, G., Mazur, A.K., Rabesona, H., Girault, J., Haertle, T., Takahashi, M., Debey, P., Hui Bon Hoa, G.

Assembly

SHEEP PRION PEPTIDE

Entity

1. SHEEP PRION PEPTIDE (polymer, Thiol state: all free), 26 monomers, 3427.695 Da Detail

GNDYEDRYYR ENMYRYPNQV YYRPVC

Formula weight

3427.695 Da

Source organism

Ovis aries

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.4 %, Completeness (bb): 98.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	99.4 % (175 of 176)	99.4 % (175 of 176)
Backbone	98.0 % (50 of 51)	98.0 % (50 of 51)
Sidechain	100.0 % (125 of 125)	100.0 % (125 of 125)
Aromatic	100.0 % (28 of 28)	100.0 % (28 of 28)
Methyl	100.0 % (4 of 4)	100.0 % (4 of 4)

1. Major Prion Protein

GNDYEDRYYR ENMYRYPNQV YYRPVC

Show sample condition

Sample

Pressure 1 atm, Temperature 278 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Major Prion Protein			4.5 mM
2	sodium phosphate			10 mM
3	H2O			90 %
4	D2O			10 %

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Calculated from 21 models in PDB: 1G04, Strand ID: A Detail

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Coupling constant

19 J values in 1 lists
Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 278 K, pH 6.5 Detail

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Pressure 1 atm, Temperature 278 K, pH 6.5

List #1 coupling_constant_set_1, Field strength (¹H) 500 MHz

Release date

2002-06-25

Citation

Sheep prion protein synthetic peptide spanning helix 1 and beta-strand 2 (residues 142-166) shows beta-hairpin structure in solution
Kozin, S.A., Bertho, G., Mazur, A.K., Rabesona, H., Girault, J., Haertle, T., Takahashi, M., Debey, P., Hui Bon Hoa, G.
J. Biol. Chem. (2001), 276, 46364-46370, PubMed 11577109 , DOI 10.1074/jbc.M108014200 , Abstract

Related entities 1. SHEEP PRION PEPTIDE, : 1 : 1 : 49 : 99 entities Detail

Interaction partners 1. SHEEP PRION PEPTIDE, : 1 interactors Detail

Experiments performed 8 experiments Detail