BMRB: 4656

Design and Solution Structure of a Well-folded Stack of Two Beta-hairpins Based on the Amino-terminal Fragment of Human Granulin A

Authors

Tolkatchev, D., Ng, A., Vranken, W., Ni, F.

Assembly

GRANULIN A

Entity

1. GRANULIN A (polymer, Thiol state: free and disulfide bound), 31 monomers, 3391.937 Da Detail

VVHCDMEVIC PDGYTCCRLP SGAWGCCPFT Q

Formula weight

3391.937 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS4:SG	1:CYS16:SG
2	disulfide	sing	1:CYS10:SG	1:CYS26:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry and simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.9 %, Completeness (bb): 98.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	95.9 % (162 of 169)	95.9 % (162 of 169)
Backbone	98.4 % (61 of 62)	98.4 % (61 of 62)
Sidechain	94.4 % (101 of 107)	94.4 % (101 of 107)
Aromatic	76.5 % (13 of 17)	76.5 % (13 of 17)
Methyl	100.0 % (13 of 13)	100.0 % (13 of 13)

1. GRANULIN A

VVHCDMEVIC PDGYTCCRLP SGAWGCCPFT Q

Show sample condition

Sample

Pressure 1 atm, Temperature 288 K, pH 5.0

#	Name	Isotope labeling	Type	Concentration
1	GRANULIN_A			0.5 mM
2	sodium acetate	[U-2H]		20 mM
3	D2O			10 %
4	H2O			90 %

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Calculated from 10 models in PDB: 1G26, Strand ID: A Detail

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Release date

2001-03-15

Citation

Identification of a thrombin-binding region in the sixth epidermal growth factor-like repeat of human thrombomodulin
Tolkatchev, D., Ng, A., Vranken, W., Ni, F.
Biochemistry (2000), 39, 10365-10372, PubMed 10956026 , DOI 10.1021/bi000715e , Abstract

Related entities 1. GRANULIN A, : 1 : 3 : 89 entities Detail

Interaction partners 1. GRANULIN A, : 50 interactors Detail

Experiments performed 1 experiments Detail