BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	70.1 % (801 of 1143)	89.1 % (531 of 596)	39.9 % (177 of 444)	90.3 % (93 of 103)
Backbone	69.6 % (402 of 578)	96.9 % (189 of 195)	42.4 % (122 of 288)	95.8 % (91 of 95)
Sidechain	63.7 % (420 of 659)	85.3 % (342 of 401)	30.4 % (76 of 250)	25.0 % (2 of 8)
Aromatic	44.1 % (30 of 68)	88.2 % (30 of 34)	0.0 % (0 of 34)
Methyl	93.6 % (103 of 110)	100.0 % (55 of 55)	87.3 % (48 of 55)

1. APOPTOTIC PROTEASE ACTIVATING FACTOR 1

MDAKARNCLL QHREALEKDI KTSYIMDHMI SDGFLTISEE EKVRNEPTQQ QRAAMLIKMI LKKDNDSYVS FYNALLHEGY KDLAALLHDG IPVVSSS

Show sample condition

Sample #1

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	[U-15N]	0.5 ~ 1.0 mM
2	phosphate buffer		20 mM
3	NaCl		50 mM
4	DTT		5 mM

Sample #2

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	[U-13C]	1 mM
6	phosphate buffer		20 mM
7	NaCl		50 mM
8	DTT		5 mM

Sample #3

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
9	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	1 mM
10	phosphate buffer	20 mM
11	NaCl	50 mM
12	DTT	5 mM

Sample #4

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
13	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	[U-13C; U-15N; U-80% 2H]	1 mM
14	phosphate buffer		20 mM
15	NaCl		50 mM
16	DTT		5 mM

Protein Blocks Logo

Calculated from 16 models in PDB: 1C15, Strand ID: A Detail

Release date

2000-03-06

Citation

Solution structure of Apaf-1 CARD and its interaction with caspase-9 CARD: a structural basis for specific adaptor/caspase interaction
Zhou, P., Chou, J., Sanchez-Olea, R., Yuan, J., Wagner, G.
Proc. Natl. Acad. Sci. U. S. A. (1999), 96, 11265-11270, PubMed 10500165 , DOI: , Abstract

Related entities 1. Apoptotic protease activating factor 1, : 1 : 12 : 4 : 13 entities Detail

Interaction partners 1. Apoptotic protease activating factor 1, : 9 interactors Detail

More details for 9 interactors identified by 7 citations

Experiments performed 4 experiments Detail

1 3D_15N-separated_NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	[U-15N]	0.5 ~ 1.0 mM
2	phosphate buffer		20 mM
3	NaCl		50 mM
4	DTT		5 mM

Sample #2

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	[U-13C]	1 mM
6	phosphate buffer		20 mM
7	NaCl		50 mM
8	DTT		5 mM

Sample #3

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
9	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	1 mM
10	phosphate buffer	20 mM
11	NaCl	50 mM
12	DTT	5 mM

Sample #4

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
13	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	[U-13C; U-15N; U-80% 2H]	1 mM
14	phosphate buffer		20 mM
15	NaCl		50 mM
16	DTT		5 mM

2 3D_13C-separated_NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	[U-15N]	0.5 ~ 1.0 mM
2	phosphate buffer		20 mM
3	NaCl		50 mM
4	DTT		5 mM

Sample #2

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	[U-13C]	1 mM
6	phosphate buffer		20 mM
7	NaCl		50 mM
8	DTT		5 mM

Sample #3

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
9	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	1 mM
10	phosphate buffer	20 mM
11	NaCl	50 mM
12	DTT	5 mM

Sample #4

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
13	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	[U-13C; U-15N; U-80% 2H]	1 mM
14	phosphate buffer		20 mM
15	NaCl		50 mM
16	DTT		5 mM

3 2D NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	[U-15N]	0.5 ~ 1.0 mM
2	phosphate buffer		20 mM
3	NaCl		50 mM
4	DTT		5 mM

Sample #2

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	[U-13C]	1 mM
6	phosphate buffer		20 mM
7	NaCl		50 mM
8	DTT		5 mM

Sample #3

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
9	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	1 mM
10	phosphate buffer	20 mM
11	NaCl	50 mM
12	DTT	5 mM

Sample #4

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
13	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	[U-13C; U-15N; U-80% 2H]	1 mM
14	phosphate buffer		20 mM
15	NaCl		50 mM
16	DTT		5 mM

4 HMQC-J

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	[U-15N]	0.5 ~ 1.0 mM
2	phosphate buffer		20 mM
3	NaCl		50 mM
4	DTT		5 mM

Sample #2

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	[U-13C]	1 mM
6	phosphate buffer		20 mM
7	NaCl		50 mM
8	DTT		5 mM

Sample #3

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
9	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	1 mM
10	phosphate buffer	20 mM
11	NaCl	50 mM
12	DTT	5 mM

Sample #4

Pressure 1 atm, Temperature 300 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
13	APOPTOTIC PROTEASE ACTIVATING FACTOR 1	[U-13C; U-15N; U-80% 2H]	1 mM
14	phosphate buffer		20 mM
15	NaCl		50 mM
16	DTT		5 mM

nullKeywords Apoptosis, Programmed cell death, APAF, CARD, DED, DD, Caspase recruitment domain, homophilic interaction

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BMRB: 4661

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Related entities 1. Apoptotic protease activating factor 1, : 1 : 12 : 4 : 13 entities Detail

Interaction partners 1. Apoptotic protease activating factor 1, : 9 interactors Detail

Experiments performed 4 experiments Detail

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Sample #4

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