BMRBj - BMREntryMaster

Found 1 Document (1.58 seconds)

BMRB: 4668

1IX5

Assignment of 1H, 13C and 15N Resonances of FKBP from Methanococcus thermolithotrophicus

Authors

Suzuki, R., Nagata, K., Kawakami, M., Nemoto, N., Furutani, M., Adachi, K., Maruyama, T., Tanokura, M.

Assembly

FK506 binding protein from Methanococcus thermolithotrophicus

Entity

1. FK506 binding protein from Methanococcus thermolithotrophicus (polymer, Thiol state: not present), 151 monomers, 16809.81 Da Detail

MVDKGVKIKV DYIGKLESGD VFDTSIEEVA KEAGIYAPDR EYEPLEFVVG EGQLIQGFEE AVLDMEVGDE KTVKIPAEKA YGNRNEMLIQ KIPRDAFKEA DFEPEEGMVI LAEGIPATIT EVTDNEVTLD FNHELAGKDL VFTIKIIEVV E

Formula weight

16809.81 Da

Source organism

Methanothermococcus thermolithotrophicus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.8 %, Completeness (bb): 98.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.8 % (1621 of 1747)	92.9 % (840 of 904)	91.2 % (630 of 691)	99.3 % (151 of 152)
Backbone	98.7 % (882 of 894)	98.7 % (304 of 308)	98.4 % (434 of 441)	99.3 % (144 of 145)
Sidechain	87.5 % (868 of 992)	89.3 % (532 of 596)	84.6 % (329 of 389)	100.0 % (7 of 7)
Aromatic	11.3 % (12 of 106)	11.3 % (6 of 53)	11.3 % (6 of 53)
Methyl	97.9 % (188 of 192)	97.9 % (94 of 96)	97.9 % (94 of 96)

1. FK506 binding protein from Methanococcus thermolithotrophicus

MVDKGVKIKV DYIGKLESGD VFDTSIEEVA KEAGIYAPDR EYEPLEFVVG EGQLIQGFEE AVLDMEVGDE KTVKIPAEKA YGNRNEMLIQ KIPRDAFKEA DFEPEEGMVI LAEGIPATIT EVTDNEVTLD FNHELAGKDL VFTIKIIEVV E

Show sample condition

Sample

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	FK506 binding protein from Methanococcus thermolithotrophicus	[U-13C; U-15N]		8.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.1 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.1 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.28 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1IX5, Strand ID: A Detail

Release date

2001-05-02

Citation

Assignment of 1H, 13C and 15N resonances of FKBP. from Methanococcus thermolithotrophicus
Suzuki, R., Nagata, K., Kawakami, M., Nemoto, N., Furutani, M., Adachi, K., Maruyama, T., Tanokura, M.
J. Biomol. NMR (2000), 17, 183-184, PubMed 10921785 , DOI: , Abstract

Related entities 1. FK506 binding protein from Methanococcus thermolithotrophicus, : 1 : 1 : 13 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	FK506 binding protein from Methanococcus thermolithotrophicus	[U-15N]		4.5 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	FK506 binding protein from Methanococcus thermolithotrophicus	[U-13C; U-15N]		8.0 mM

2 3D 1H-1H-15N NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	FK506 binding protein from Methanococcus thermolithotrophicus	[U-15N]		4.5 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	FK506 binding protein from Methanococcus thermolithotrophicus	[U-13C; U-15N]		8.0 mM

3 3D 1H-1H-15N TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	FK506 binding protein from Methanococcus thermolithotrophicus	[U-15N]		4.5 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	FK506 binding protein from Methanococcus thermolithotrophicus	[U-13C; U-15N]		8.0 mM

4 3D HNCA

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	FK506 binding protein from Methanococcus thermolithotrophicus	[U-15N]		4.5 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	FK506 binding protein from Methanococcus thermolithotrophicus	[U-13C; U-15N]		8.0 mM

5 3D HNCACB

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	FK506 binding protein from Methanococcus thermolithotrophicus	[U-15N]		4.5 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	FK506 binding protein from Methanococcus thermolithotrophicus	[U-13C; U-15N]		8.0 mM

6 3D CBCA(CO)NH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	FK506 binding protein from Methanococcus thermolithotrophicus	[U-15N]		4.5 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	FK506 binding protein from Methanococcus thermolithotrophicus	[U-13C; U-15N]		8.0 mM

7 3D HNCO

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	FK506 binding protein from Methanococcus thermolithotrophicus	[U-15N]		4.5 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	FK506 binding protein from Methanococcus thermolithotrophicus	[U-13C; U-15N]		8.0 mM

8 3D (HCA)CO(CA)NH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	FK506 binding protein from Methanococcus thermolithotrophicus	[U-15N]		4.5 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	FK506 binding protein from Methanococcus thermolithotrophicus	[U-13C; U-15N]		8.0 mM

9 3D H(CCO)NH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	FK506 binding protein from Methanococcus thermolithotrophicus	[U-15N]		4.5 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	FK506 binding protein from Methanococcus thermolithotrophicus	[U-13C; U-15N]		8.0 mM

10 3D C(CO)NH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	FK506 binding protein from Methanococcus thermolithotrophicus	[U-15N]		4.5 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	FK506 binding protein from Methanococcus thermolithotrophicus	[U-13C; U-15N]		8.0 mM

11 3D 1H-13C-1H DE-H(C)CH-TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	FK506 binding protein from Methanococcus thermolithotrophicus	[U-15N]		4.5 mM

Sample #2

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	FK506 binding protein from Methanococcus thermolithotrophicus	[U-13C; U-15N]		8.0 mM

nullKeywords PPIase, FKBP, thermophilic archaeon, NMR assignments

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Found 1 Document (1.58 seconds)

BMRB: 4668

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. FK506 binding protein from Methanococcus thermolithotrophicus, : 1 : 1 : 13 entities Detail

Experiments performed 11 experiments Detail

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