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BMRB: 4682


4682
1EII
Backbone and side-chain 1H, 13C, and 15N Chemical Shift Assignments for holo-CRBP II
Authors
Lu, J., Lin, C., Tang, C., Ponder, J.W., Kao, J.L.F., Cistola, D.P., Li, E.
Assembly
Rat holo-cellular retinol-binding protein II
Entity
1. Holo cellular retinol-binding protein II (polymer, Thiol state: all free), 134 monomers, 15584.38 Da Detail

MTKDQNGTWE MESNENFEGY MKALDIDFAT RKIAVRLTQT KIIVQDGDNF KTKTNSTFRN YDLDFTVGVE FDEHTKGLDG RNVKTLVTWE GNTLVCVQKG EKENRGWKQW VEGDKLYLEL TCGDQVCRQV FKKK


2. RTL (non-polymer), 286.452 Da
Total weight
15870.832 Da
Max. entity weight
15584.38 Da
Source organism
Rattus norvegicus
Exptl. method
NMR
Refine. method
distance geometry & simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 23.9 %, Completeness: 17.3 %, Completeness (bb): 21.0 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All17.3 % (279 of 1612)16.2 % (136 of 842)17.9 % (110 of 616)21.4 % (33 of 154)
Backbone21.0 % (169 of 804)21.5 % (60 of 279)19.9 % (78 of 391)23.1 % (31 of 134)
Sidechain14.3 % (133 of 931)13.5 % (76 of 563)15.8 % (55 of 348)10.0 % (2 of 20)
Aromatic15.1 % (22 of 146)15.1 % (11 of 73)14.5 % (10 of 69)25.0 % (1 of 4)
Methyl18.5 % (24 of 130)20.0 % (13 of 65)16.9 % (11 of 65)

1. Holo cellular retinol-binding protein II

MTKDQNGTWE MESNENFEGY MKALDIDFAT RKIAVRLTQT KIIVQDGDNF KTKTNSTFRN YDLDFTVGVE FDEHTKGLDG RNVKTLVTWE GNTLVCVQKG EKENRGWKQW VEGDKLYLEL TCGDQVCRQV FKKK

Show sample condition
Sample #1

Temperature 298 (±1) K, pH 7.4 (±0.2)


#NameIsotope labelingTypeConcentration
1Holo cellular retinol-binding protein II[U-99% 13C; U-99% 15N]1.0 ~ 2.0 mM
Sample #2

Temperature 298 (±1) K, pH 7.4 (±0.2)


#NameIsotope labelingTypeConcentration
2Holo cellular retinol-binding protein II1.5 mM

Chem. Shift Complete2
Sequence coverage: 99.3 %, Completeness: 54.9 %, Completeness (bb): 59.7 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All54.9 % (1771 of 3224)56.1 % (944 of 1684)52.2 % (643 of 1232)59.7 % (184 of 308)
Backbone59.7 % (960 of 1608)59.9 % (334 of 558)59.2 % (463 of 782)60.8 % (163 of 268)
Sidechain51.3 % (955 of 1862)54.2 % (610 of 1126)46.6 % (324 of 696)52.5 % (21 of 40)
Aromatic31.8 % (93 of 292)53.4 % (78 of 146) 7.2 % (10 of 138)62.5 % (5 of 8)
Methyl59.2 % (154 of 260)60.0 % (78 of 130)58.5 % (76 of 130)

1. Holo cellular retinol-binding protein II

MTKDQNGTWE MESNENFEGY MKALDIDFAT RKIAVRLTQT KIIVQDGDNF KTKTNSTFRN YDLDFTVGVE FDEHTKGLDG RNVKTLVTWE GNTLVCVQKG EKENRGWKQW VEGDKLYLEL TCGDQVCRQV FKKK

Show sample condition
Sample #1

Temperature 298 (±1) K, pH 7.4 (±0.2)


#NameIsotope labelingTypeConcentration
1Holo cellular retinol-binding protein II[U-99% 13C; U-99% 15N]1.0 ~ 2.0 mM
Sample #2

Temperature 298 (±1) K, pH 7.4 (±0.2)


#NameIsotope labelingTypeConcentration
2Holo cellular retinol-binding protein II1.5 mM

Chem. Shift Complete3
Sequence coverage: 98.5 %, Completeness: 53.7 %, Completeness (bb): 60.3 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All53.7 % (2596 of 4836)59.1 % (1492 of 2526)41.6 % (769 of 1848)72.5 % (335 of 462)
Backbone60.3 % (1455 of 2412)72.2 % (604 of 837)47.4 % (556 of 1173)73.4 % (295 of 402)
Sidechain47.2 % (1317 of 2793)52.6 % (888 of 1689)37.3 % (389 of 1044)66.7 % (40 of 60)
Aromatic26.5 % (116 of 438)42.0 % (92 of 219) 7.2 % (15 of 207)75.0 % (9 of 12)
Methyl52.8 % (206 of 390)57.9 % (113 of 195)47.7 % (93 of 195)

1. Holo cellular retinol-binding protein II

MTKDQNGTWE MESNENFEGY MKALDIDFAT RKIAVRLTQT KIIVQDGDNF KTKTNSTFRN YDLDFTVGVE FDEHTKGLDG RNVKTLVTWE GNTLVCVQKG EKENRGWKQW VEGDKLYLEL TCGDQVCRQV FKKK

Show sample condition
Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)


#NameIsotope labelingTypeConcentration
3Holo cellular retinol-binding protein II[U-99% 15N]1.5 mM

Protein Blocks Logo
Calculated from 25 models in PDB: 1EII, Strand ID: A Detail

Release date
2000-08-02
Citation 1
Binding of retinol induces changes in rat cellular retinol-binding protein II conformation and backbone dynamics
Lu, J., Lin, C., Tang, C., Ponder, J.W., Kao, J.L.F., Cistola, D.P., Li, E.
J. Mol. Biol. (2000), 300, 619-632, PubMed 10884357 , DOI 10.1006/jmbi.2000.3883 ,
Show more 1 citaion
Citation 2
Crystal structures of holo and apo-cellular retinol-binding protein II
Winter, N.S., Bratt, J.M., Banaszak, L.J.
J. Mol. Biol. (1993), 230, 1247-1259, PubMed 8487303 , DOI 10.1006/jmbi.1993.1239 ,
Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail
  BMRB: 4681 released on 2000-03-19
    Title Backbone and side-chain 1H, 13C, and 15N Chemical Shift Assignments for apo-CRBPII
  Swiss-Prot: P06768 released on 1988-01-01
    Title RET2_RAT Entity Retinol-binding protein 2
Related entities 1. Holo cellular retinol-binding protein II, : 1 : 5 : 252 entities Detail
More details for 258 related entities
Interaction partners 1. Holo cellular retinol-binding protein II, : 1 interactors Detail
More details for 1 interactors identified by 1 citations
nullKeywords cellular retinol-binding protein, lipid-binding protein, NMR, structure, lipid transport